Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
Blanco Prieto, Olga (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Maside, Carolina (Universitat de Girona. Departament de Biologia)
Gruzmacher, Andrea (Universidad Austral de Chile)
Ortiz, Manuel (Universidad Austral de Chile)
Ratto, Marcelo (Universidad Austral de Chile)
Urra, Francisco Javier (Universidad Austral de Chile)
Vera, Tomás (Universidad Austral de Chile)
Strobel, Pablo (Universidad Austral de Chile)
Catalán, Jaime (Universitat de Girona. Departament de Biologia)
Mislei, Beatrice (Università di Bologna)
Bucci, Diego (Università di Bologna)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ramírez-Reveco, Alfredo (Universidad Austral de Chile)

Date:	2025
Abstract:	This work sought to test the positive effect of red LED light on frozen bovine sperm resistance to thawing in suboptimal conditions. Moreover, a preliminary study explores whether this improvement could have any repercussions on in vivo fertilizing ability. Thus, frozen bull semen straws (n = 16) were thawed (a) with irradiation for 5 min at 20 °C (2 min light, 1 min darkness, 2 min light; PHOTO); (b) without irradiation for 5 min at 20 °C (ET); or (c) through immersion into a water bath at 38 °C for 40 s as standard control (CONTROL). Sperm quality and preliminary, purely descriptive AI trials were performed. The PHOTO samples demonstrated significantly (p < 0. 05) improved percentages of intact acrosomes, progressive motility, DNA condensation and fragmentation, and viable sperm with high ROS/superoxides. The viability of PHOTO samples decreased significantly (p < 0. 05) when compared with the ET ones. Overall results of both the PHOTO and ET samples were poorer than those of the CONTROL. Otherwise, the PHOTO straws yielded greater pregnancy rates (64. 0% vs. 49. 4% in CONTROL) when evaluated at two different farms. The results suggest that irradiating cryopreserved bovine sperm during thawing in suboptimal conditions could improve AI pregnancy rates, although more in vivo studies are needed to support this conclusion.
Grants:	Agencia Estatal de Investigación AGL2017-88329-RMCIN/AGL2017-88329-R Agencia Estatal de Investigación PID2020-113320RB-I00MCIN/PID2020-113320RB-I00 Generalitat de Catalunya 2017-SGR-1229Generalitat de Catalunya/2017-SGR-1229
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Red light ; Cryopreserved sperm ; Artificial insemination
Published in:	Animals, Vol. 15, Num. 22 (November 2025) , art. 3353, ISSN 2076-2615

DOI: 10.3390/ani15223353
PMID: 41302061

25 p, 1.8 MB

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