The Interplay Between Disordered Regions in RNAs and Proteins Modulates Interactions Within Stress Granules and Processing Bodies.
Vandelli, Andrea (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Cid Samper, Fernando (Universitat Pompeu Fabra)
Torrent, Marc (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Sánchez de Groot, Natalia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Tartaglia, Gian Gaetano (Institut de Ciència i Tecnologia de Barcelona)

Date:	2021
Abstract:	Condensation, or liquid-like phase separation, is a phenomenon indispensable for the spatiotemporal regulation of molecules within the cell. Recent studies indicate that the composition and molecular organization of phase-separated organelles such as Stress Granules (SGs) and Processing Bodies (PBs) are highly variable and dynamic. A dense contact network involving both RNAs and proteins controls the formation of SGs and PBs and an intricate molecular architecture, at present poorly understood, guarantees that these assemblies sense and adapt to different stresses and environmental changes. Here, we investigated the physico-chemical properties of SGs and PBs components and studied the architecture of their interaction networks. We found that proteins and RNAs establishing the largest amount of contacts in SGs and PBs have distinct properties and intrinsic disorder is enriched in all protein-RNA, protein-protein and RNA-RNA interaction networks. The increase of disorder in proteins is accompanied by an enrichment in single-stranded regions of RNA binding partners. Our results suggest that SGs and PBs quickly assemble and disassemble through dynamic contacts modulated by unfolded domains of their components.
Grants:	European Commission 309545 European Commission 855923 European Commission 727658 Agencia Estatal de Investigación RYC2019-026752-I European Commission 825080 Agencia Estatal de Investigación PID2020-117454RA-I00
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Structural disorder ; Interaction networks ; Liquid-liquid phase separation ; Fuzzy interactions ; RNA and proteins
Published in:	Journal of Molecular Biology, Vol. 434, Num. 1 (January 2022) , art. 167159, ISSN 1089-8638

DOI: 10.1016/j.jmb.2021.167159
PMID: 34274326

12 p, 739.1 KB

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