Bariatric Surgery Reverses ORG and Exhibits a Distinct Transcriptomic Profile Compared to Weight Loss Through a Low-Fat Diet
López-Martínez, Marina (Universitat Autònoma de Barcelona)
Rodriguez Martinez, Paula (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Blay, Lidia (Universitat Autònoma de Barcelona. Departament de Cirurgia)
Armengol Barnils, Maria del Pilar (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pey, Irina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ferrer, Mireia (Vall d'Hebron Institut de Recerca (VHIR))
Porrini, Esteban (Universidad de La Laguna)
Luis-Lima, Sergio (Universidad de La Laguna)
Díaz-Martín, Laura (Complejo Hospitalario Universitario de Canarias)
Rodríguez-Rodríguez, Ana Elena (Hospital Universitario de Canarias (La Laguna))
Cruz-Perera, Coriolano (Complejo Hospitalario Universitario de Canarias)
Navarro Diaz, Maruja Isabel (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)

Fecha:	2026
Resumen:	Weight loss is central to treating obesity-related kidney disease, yet the renal effects of a low-fat diet (LFD) versus bariatric surgery (BS) remain incompletely understood. This study compared their impact on obesity-related glomerulopathy (ORG). Twenty-eight male Wistar rats were fed a high-fat diet (HFD) for 10 weeks to induce obesity. Eight rats were sacrificed (the HFD group), eight switched to LFD for 10 weeks, and twelve underwent sleeve gastrectomy. Body weight, albuminuria, renal histology, and transcriptomic profiles were analyzed. Weight loss was modest in the LFD group (-1. 6%) but substantial after BS (-13. 2%), occurring 2. 1 times faster. Albuminuria decreased in both interventions compared to HFD (LFD: 7228 ± 514 ng/mL; BS: 6242 ± 418 ng/mL; HFD: 10,384 ± 1168 ng/mL; p < 0. 01) and correlated strongly with weight loss (R = 0. 78). The glomerular area was reduced in both groups, but only BS achieved complete histological resolution of ORG. Tubular cells in BS-treated rats showed megamitochondria and cristae disruption, while LFD induced milder alterations. Transcriptomics revealed suppression of mitochondrial maintenance genes and upregulation of oxidative stress and immunometabolic pathways. Immune-related genes upregulated in BS clustered into pro-inflammatory/chemotactic and regulatory modules. To the best of our knowledge, this is the first piece of evidence that BS fully reverses ORG, highlighting renal effects beyond weight loss alone.
Ayudas:	Instituto de Salud Carlos III PI18-01951
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Obesity-related glomerulopathy ; Bariatric surgery ; Low-fat diet ; Obesity glomerulopathy reversion ; Transcriptomic changes ; Wistar rats
Publicado en:	International journal of molecular sciences, Vol. 27, Num. 2 (January 2026) , p. 839, ISSN 1422-0067

DOI: 10.3390/ijms27020839

17 p, 21.1 MB

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