Characterization of Infarct Size and Remodeling Using CMR and PET in Mice Models of Reperfused and Non-Reperfused Myocardial Infarction
Gavara Doñate, Jose (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Molina-Garcia, Tamara (Universitat de València)
Ezzeddin, Mustafa (Universitat de València)
Diaz, Ana (Universitat de València)
Perez-Solé, Nerea (Centro de Investigación Biomédica en Red-Cardiovascular (Madrid))
Ortega, Maria (INCLIVA Biomedical Research Institute)
Marcos-Garces, Victor (Hospital Clínic Universitari (València))
de Dios, Elena (Centro de Investigación Biomédica en Red-Cardiovascular (Madrid))
Bayés-Genís, Antoni (Universitat Autònoma de Barcelona. Departament de Medicina)
Ruiz-Sauri, Amparo (Universitat de València)
Rios-Navarro, Cesar (Universitat de València)
Bodi, Vicente (Hospital Clínic Universitari (València))

Date:	2025
Abstract:	Background/Objectives : Unlike post-mortem histopathology, cardiovascular magnetic resonance (CMR) and positron emission tomography (PET) enable longitudinal assessment of structural, functional, and metabolic alterations in preclinical myocardial infarction models. This study aims to describe the temporal evolution of infarct size and systolic function by CMR and glucose consumption via PET, explore their differences in non-reperfused and reperfusion infarction models, and assess their capacity to predict histology-derived infarct size and systolic function at chronic phase CMR. Methods : Two murine models of myocardial infarction were generated using permanent (non-reperfused, n = 8) or transient (reperfused, n = 9) coronary occlusion. CMR and fluorine-18 2-fluoro-2-deoxyglucose PET imaging were performed at baseline and at 1, 7, and 21 days post-infarction to quantify infarct size, systolic function, and myocardial glucose metabolism. Infarct size was also assessed using Masson's trichrome staining. Results : At 24 h post-infarction, CMR-derived infarction together with significant reduction in systolic function and glucose metabolism were already noted in both models. At 21-day CMR, however, reperfused mice showed lower infarct size and more preserved systolic function compared to their non-reperfused counterparts, while no differences in glucose metabolism were reported. Infarct size and systolic function at 1-day CMR and the number of segments with reduced glucose consumption at 1-day PET independently predicted histology-derived infarct size and long-term systolic function. Conclusions : Combined PET/CMR imaging enables non-invasive, sequential evaluation of infarct size, systolic function, and glucose metabolism in experimental myocardial infarction. This multimodality approach is well suited for assessing the efficacy of emerging therapies in preclinical research.
Grants:	Instituto de Salud Carlos III PI23/01150 Ministerio de Economía y Competitividad 16/11/00403 Ministerio de Economía y Competitividad 16/11/00486
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Magnetic resonance ; Myocardial infarction ; Positron emission tomography
Published in:	Diagnostics, Vol. 15, Num. 23 (November 2025) , art. 2960, ISSN 2075-4418

DOI: 10.3390/diagnostics15232960
PMID: 41374341

20 p, 4.1 MB

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