An in silico analysis of laccase thermostability determinants for the improvement of rational enzyme engineering
Salarich Soldevila, Adrià
Parladé, Eloi tut. (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Universitat Autònoma de Barcelona. Facultat de Biociències

Data:	2026
Resum:	Laccases (EC:1. 10. 3. 2) are enzymes of the multi-copper oxidase family capable of degrading a wide range of substrates using oxygen as the final electron acceptor and producing water as a by-product of the reaction. Owing to this characteristic, they are promising tools in the field of bioremediation. However, the application of laccases in large-scale procedures is hindered by the low thermostability of many of these enzymes, a property whose molecular determinants remain to be fully characterised. Therefore, the aim of this project was to analyse thermostable and non-thermostable laccases to elucidate their physicochemical differences and identify key residues contributing to thermostability. To achieve this, multiple-sequence alignments of thermostable and non-thermostable laccases were constructed and analysed through statistical tests and the Kullback-Leibler divergence. The analysis revealed a high degree of global similarity between the two groups of enzymes, suggesting that thermostability is mostly determined by local conservation patterns. Using the Kullback-Leibler divergence and Bacillus subtilis CotA structure as a reference, several discriminating positions were identified and structurally interpreted, revealing conservation patterns with a potential to be used in the targeted rational design of laccases to increase their thermostability.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre i quan aquestes es distribueixin sota la mateixa llicència que regula l'obra original i es reconegui l'autoria.
Llengua:	Anglès
Titulació:	Microbiologia [3602]
Pla d'estudis:	Grau en Microbiologia [816]
Document:	Treball final de grau
Matèria:	Bacterial laccase ; Thermostability ; Bioremediation ; Rational engineering ; Multiple-sequence alignment (MSA)

Pòster 1 p, 4.7 MB

TFG 24 p, 7.3 MB

El registre apareix a les col·leccions:
Documents de recerca > Treballs de final de grau > Facultat de Biociències. TFG