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12 p, 1.6 MB Direct Quantitative Immunochemical Analysis of Autoinducer Peptide IV for Diagnosing and Stratifying Staphylococcus aureus Infections / Montagut, Enrique-J. (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) ; Acosta, Gerardo (Institut de Química Avançada de Catalunya) ; Albericio, Fernando (University of KwaZulu-Natal) ; Royo, Miriam (Institut de Química Avançada de Catalunya) ; Godoy-Tena, Gerard (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Lacoma, Alicia (Centro de Investigación Biomédica en Red de Enfermedades Respiratorias) ; Prat i Aymerich, Cristina (University Medical Center Utrecht, Utrecht University) ; Salvador, Juan-Pablo (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) ; Marco, María Pilar (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
An immunochemical strategy to detect and quantify AIP-IV, the quorum sensing (QS) signaling molecule produced by Staphylococcus aureus agr type IV, is reported here for the first time. Theoretical calculations and molecular modeling studies have assisted on the design and synthesis of a suitable peptide hapten (AIPIVS), allowing to obtain high avidity and specific antibodies toward this peptide despite its low molecular weight. [...]
2022 - 10.1021/acsinfecdis.1c00670
ACS infectious diseases, Vol. 8 (02 2022) , p. 645-656  

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