Pérez-Gil, Jordi - Resultats de la cerca - Dipòsit Digital de Documents de la UAB

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12 p, 1.7 MB

MEP pathway products allosterically promote monomerization of deoxy-D-xylulose-5-phosphate synthase to feedback-regulate their supply / Di, Xueni (Centre de Recerca en Agrigenòmica) ; Ortega-Alarcon, David (Universidad de Zaragoza. Departamento de Bioquímica y Biología Molecular y Celular) ; Kakumanu, Ramu (Lawrence Berkeley National Laboratory) ; Iglesias-Fernandez, Javier (Nostrum Biodiscovery) ; Diaz, Lucia (Nostrum Biodiscovery) ; Baidoo, Edward E.K. (Lawrence Berkeley National Laboratory) ; Velázquez-Campoy, Adrian (Universidad de Zaragoza. Departamento de Bioquímica y Biología Molecular y Celular) ; Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica) ; Pérez-Gil, Jordi (Centre de Recerca en Agrigenòmica)
Isoprenoids are a very large and diverse family of metabolites required by all living organisms. All isoprenoids derive from the double-bond isomers isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are produced by the methylerythritol 4-phosphate (MEP) pathway in bacteria and plant plastids. [...]
2023 - DOI (Digital Object Identifier)

10.1016/j.xplc.2022.100512
Plant Communications, Vol. 4, Issue 3 (May 2023) , art. 100512

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16 p, 1.5 MB

Adaptation of hydroxymethylbutenyl diphosphate reductase enables volatile isoprenoid production / Bongers, Mareike (The University of Queensland. Australian Institute for Bioengineering and Nanotechnolog (Australia)) ; Pérez-Gil, Jordi (Centre de Recerca en Agrigenòmica) ; Hodson, Mark P. (The University of Queensland. Australian Institute for Bioengineering and Nanotechnolog (Australia)) ; Schrübbers, Lars (Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability (Denmark)) ; Wulff, Tune (Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability (Denmark)) ; Sommer, Morten O. A. (Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability (Denmark)) ; Nielsen, Lars K. (The University of Queensland. Australian Institute for Bioengineering and Nanotechnolog (Australia)) ; Vickers, Claudia E. (CSIRO Synthetic Biology Future Science Platform (Australia))
Volatile isoprenoids produced by plants are emitted in vast quantities into the atmosphere, with substantial effects on global carbon cycling. Yet, the molecular mechanisms regulating the balance between volatile and non-volatile isoprenoid production remain unknown. [...]
2020 - DOI (Digital Object Identifier)

10.7554/eLife.48685
eLife, Vol. 9 (March 2020) , art. e48685

2 documents

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14 p, 1.7 MB

Engineering Pseudomonas putida for isoprenoid production by manipulating endogenous and shunt pathways supplying precursors / Hernandez-Arranz, Sofia (Centre de Recerca en Agrigenòmica) ; Pérez-Gil, Jordi (Centre de Recerca en Agrigenòmica) ; Marshall-Sabey, D. (Centre de Recerca en Agrigenòmica) ; Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica)
Background: The soil bacterium Pseudomonas putida is a promising platform for the production of industrially valuable natural compounds. In the case of isoprenoids, the availability of biosynthetic precursors is a major limiting factor. [...]
2019 - DOI (Digital Object Identifier)

10.1186/s12934-019-1204-z
Microbial cell factories, Vol. 18 (September 2019) , art. 152

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6 p, 558.0 KB

Mutations in Escherichia coli aceE and ribB genes allow survival of strains defective in the first step of the isoprenoid biosynthesis pathway / Pérez-Gil, Jordi (Centre de Recerca en Agrigenòmica) ; Uros, Eva Maria (Centre de Recerca en Agrigenòmica) ; Sauret-Güeto, Susanna (Centre de Recerca en Agrigenòmica) ; Lois, L. Maria (Centre de Recerca en Agrigenòmica) ; Kirby, James (Joint BioEnergy Institute) ; Nishimoto, Minobu (Joint BioEnergy Institute) ; Baidoo, Edward E. K. (Joint BioEnergy Institute) ; Keasling, Jay D. (Joint BioEnergy Institute) ; Boronat i Margosa, Albert (Centre de Recerca en Agrigenòmica) ; Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica)
A functional 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is required for isoprenoid biosynthesis and hence survival in Escherichia coli and most other bacteria. In the first two steps of the pathway, MEP is produced from the central metabolic intermediates pyruvate and glyceraldehyde 3-phosphate via 1-deoxy-D-xylulose 5-phosphate (DXP) by the activity of the enzymes DXP synthase (DXS) and DXP reductoisomerase (DXR). [...]
2012 - DOI (Digital Object Identifier)

10.1371/journal.pone.0043775
PloS one, Vol. 7, issue 8 (Aug. 2012) , e43775

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18 p, 2.0 MB

Evolutionary diversification and characterization of the eubacterial gene family encoding DXR type II, an alternative isoprenoid biosynthetic enzyme / Carretero-Paulet, Lorenzo (Instituto de Biologia Molecular y Celular de Plantas (València, Espanya)) ; Lipska, Agnieszka (Instituto de Biologia Molecular y Celular de Plantas (València, Espanya)) ; Pérez-Gil, Jordi (Centre de Recerca en Agrigenòmica) ; Sangari, Félix J. (Universidad de Cantabria. Departamento de Biología Molecular) ; Albert, Victor A. (Instituto de Biologia Molecular y Celular de Plantas (València, Espanya)) ; Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica)
Background: isoprenoids constitute a vast family of natural compounds performing diverse and essential functions in all domains of life. In most eubacteria, isoprenoids are synthesized through the methylerythritol 4-phosphate (MEP) pathway. [...]
2013 - DOI (Digital Object Identifier)

10.1186/1471-2148-13-180
BMC evolutionary biology, Vol. 13 (2013) , art. 180

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