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8 p, 4.1 MB Cofactor Binding Dynamics Influence the Catalytic Activity and Selectivity of an Artificial Metalloenzyme / Villarino, Lara (University of Groningen) ; Chordia, Shreyans (University of Groningen) ; Alonso-Cotchico, Lur (University of Groningen) ; Reddem, Eswar (University of Groningen) ; Zhou, Zhi (University of Groningen) ; Thunnissen, Andy Mark W. H. (University of Groningen) ; Maréchal, Jean-Didier (Universitat Autònoma de Barcelona. Departament de Química) ; Roelfes, Gerard (University of Groningen)
We present an artificial metalloenzyme based on the transcriptional regulator LmrR that exhibits dynamics involving the positioning of its abiological metal cofactor. The position of the cofactor, in turn, was found to be related to the preferred catalytic reactivity, which is either the enantioselective Friedel-Crafts alkylation of indoles with β-substituted enones or the tandem Friedel-Crafts alkylation/enantioselective protonation of indoles with α-substituted enones. [...]
2020 - 10.1021/acscatal.0c01619
ACS catalysis, Vol. 10, Num 20 (2020) , p. 11783-11790  

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