Genetic diversity in Spanish donkey breeds using microsatellite DNA markers
Aranguren Méndez, José Atilio (Universidad del Zulia. Departamento de Producción Animal (Maracaibo, Veneçuela))
Jordana i Vidal, Jordi (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Gómez, Mariano (Diputación Foral de Bizkaia. Servicio de Ganadería)

Date:	2001
Abstract:	Genetic diversity at 13 equine microsatellite loci was compared in five endangered Spanish donkey breeds: Andaluza, Catalana, Mallorquina, Encartaciones and Zamorano-Leonesa. All of the equine microsatellites used in this study were amplified and were polymorphic in the domestic donkey breeds with the exception of HMS1, which was monomorphic, and ASB2, which failed to amplify. Allele number, frequency distributions and mean heterozygosities were very similar among the Spanish donkey breeds. The unbiased expected heterozygosity (HE) over all the populations varied between 0. 637 and 0. 684 in this study. The low GST value showed that only 3. 6% of the diversity was between breeds (P < 0. 01). Significant deviations from Hardy-Weinberg equilibrium were shown for a number of locus-population combinations, except HMS5 that showed agreement in all analysed populations. The cumulative exclusion probability (PE) was 0. 999 in each breed, suggesting that the loci would be suitable for donkey parentage testing. The constructed dendrogram from the DA distance matrix showed little differentiation between Spanish breeds, but great differentiation between them and the Moroccan ass and also with the horse, used as an outgroup. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in domestic donkey populations, which could also be useful for conservation plans.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; Versió publicada
Subject:	Donkey ; Endangered breed ; Microsatellite ; Diversity ; Genetic variability
Published in:	Genetics, selection, evolution, Vol. 33 (July 2001) , p. 433-442, ISSN 1297-9686

DOI: 10.1186/1297-9686-33-4-433
PMID: 11559485

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