Cellular distribution of the histamine H3 receptor in the basal ganglia : functional modulation of dopamine and glutamate neurotransmission
González Sepúlveda, Marta (Universitat Autònoma de Barcelona. Institut de Neurociències)
Rosell Vilar, Santi (Universitat Autònoma de Barcelona. Institut de Neurociències)
Hoffmann, Hanne Mette (University of Montpellier II)
Castillo-Ruiz, Mª del Mar (Universitat Autònoma de Barcelona. Institut de Neurociències)
Mignon, Virginie (Université Paris Descartes)
Moreno Delgado, David (Universitat Autònoma de Barcelona. Institut de Neurociències)
Vignes, Michel (University of Montpellier II)
Díaz, Jorge (Université Paris Descartes)
Sabrià i Pau, Josefa (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Ortiz de Pablo, Jordi (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Fecha:	2013
Resumen:	Histamine H3 receptors (H3R) are widely expressed in the brain where they participate in sleep-wake cycle and cognition among other functions. Despite their high expression in some regions of the basal ganglia, their functional role in this forebrain neural network remains unclear. The present findings provide in situ hybridization and immunohistochemical evidence for H3R expression in several neuronal populations of the rat basal ganglia but not in astrocytes (glial fibrillary acidic protein immunoreactive cells). We demonstrate the presence of H3R mRNA and protein in dopaminergic neurons (tyrosine hydroxylase positive) of the ventral tegmental area and substantia nigra. In the dorsal and ventral (nucleus accumbens) striatal complex we show H3R immunoreactivity in cholinergic (choline acetyltransferase immunoreactive) and GABAergic neurons (substance P, proenkephalin or dopamine D1 receptor positive) as well as in corticostriatal terminals (VGLUT1-immunoreactive). Double-labelling experiments in the medial prefrontal cortex show that H3R is expressed in D1R-positive interneurons and VGLUT1-positive corticostriatal output neurons. Our functional experiments confirm that H3R ligands modulate dopamine synthesis and the probability of glutamate release in the striatum from cortico-striatal afferents. The presence of H3R in such different neuronal populations and its involvement in the control of striatal dopaminergic and glutamatergic transmission ascribes a complex role to H3R in the function of the basal ganglia neural network.
Ayudas:	Ministerio de Educación y Ciencia SAF2006-08240 Ministerio de Educación y Ciencia SAF2009-12510
Nota:	This is the author's version of a work that was accepted for publication in Basal ganglia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Vol. 3 Núm. 2 (Jul. 2013)
Nota:	Altres ajuts: Red_de_Trastornos_Adictivos/RD06/0001/0015
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Histamine H3 receptor ; Dopamine ; Electrophysiology ; Colocalization ; Receptor d'histamina H3 ; Dopamina ; Electrofisiologia ; Colocalització
Publicado en:	Basal ganglia, Vol. 3 No. 2 (Jul. 2013) , p. 109-121, ISSN 2210-5336

DOI: 10.1016/j.baga.2012.12.001

Post-print 27 p, 5.0 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut de Neurociències (INc)
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