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Pàgina inicial > Articles > Articles publicats > DNA polymorphism sensitive impedimetric detection on gold-nanoislands modified electrodes |
Data: | 2015 |
Resum: | Nanocomposite materials are being increasingly used in biosensing applications as they can significantly improve biosensor performance. Here we report the use of a novel impedimetric genosensor based on gold nanoparticles graphite-epoxy nanocomposite (nanoAu-GEC) for the detection of triple base mutation deletion in a cystic-fibrosis (CF) related human DNA sequence. The developed platform consists of chemisorbing gold nano-islands surrounded by rigid, non-chemisorbing, and conducting graphite-epoxy composite. The ratio of the gold nanoparticles in the composite was carefully optimized by electrochemical and microscopy studies. Such platform allows the very fast and stable thiol immobilization of DNA probes on the gold islands, thus minimizing the steric and electrostatic repulsion among the DNA probes and improving the detection of DNA polymorphism down to 2. 25 fmol by using electrochemical impedance spectroscopy. These findings are very important in order to develop new and renewable platforms to be used in point-of-care devices for the detection of biomolecules. |
Ajuts: | Ministerio de Economía y Competitividad CTQ2013-41577-P Ministerio de Economía y Competitividad BIO2010-17566 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Matèria: | Gold nanocomposite ; DNA polymorphism ; Electrochemical impedance spectroscopy ; Cyctic fibrosis ; Gold nanoparticles amplification |
Publicat a: | Talanta, Vol. 136 (May 2015) , p. 95-101, ISSN 1873-3573 |
Post-print 23 p, 6.5 MB |