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Web of Science: 15 cites,
Predicted Peptides from Non-Structural Proteins of Porcine Reproductive and Respiratory Syndrome Virus Are Able to Induce IFN-γ and IL-10
Burgara Estrella, Alexel (Centro de Investigación en Alimentación y Desarrollo (Sonora, Mèxic))
Díaz Luque, Ivan (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Rodríguez Gómez, Irene (Fundació Centre de Recerca en Sanitat Animal)
Essler, Sabine E. (University of Veterinary Medicine Vienna)
Hernández, Jesús (Centro de Investigación en Alimentación y Desarrollo (Sonora, Mèxic))
Mateu de Antonio, Enrique María (Universitat Autònoma de Barcelona. Departament de Sanitat i Anatomia Animals)

Data: 2013
Resum: This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-γ and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened for IFN-γ responses (ELISPOT) and three peptides were identified; two of them in non-conserved segments of nsp2 and nsp5 and the other in a conserved region of nsp5 peptide. Then, peptides were screened for IL-10 inducing properties. Six peptides were found to induce IL-10 release in PBMC and some of them were also able to inhibit IFN-γ responses on PHA-stimulated cells. Interestingly, the IFN-γ low responder pigs against PRRSV were mostly homozygous for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV contain T-cell epitopes inducing IFN-γ responses as well as IL-10 inducing segments with inhibitory capabilities.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: PRRSV ; Non-structural proteins ; Epitopes ; Interferon gamma ; IL-10
Publicat a: Viruses, Vol. 5 (2013) , p. 663-677, ISSN 1999-4915

DOI: 10.3390/v5020663
PMID: 23435238

15 p, 355.2 KB

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