Study of protein expresion in peri-infarct tissue after cerebral ischemia
Brea, David (Instituto de Investigación Sanitaria de Santiago (IDIS))
Agulla, Jesús (Instituto de Investigación Sanitaria de Santiago (IDIS))
Staes, An (Ghent University)
Gevaert, Kris (Ghent University)
Campos, Francisco (Instituto de Investigación Sanitaria de Santiago (IDIS))
Sobrino, Tomás (Instituto de Investigación Sanitaria de Santiago (IDIS))
Blanco, Miguel (Instituto de Investigación Sanitaria de Santiago (IDIS))
Dávalos, Antoni (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Castillo, José (Instituto de Investigación Sanitaria de Santiago (IDIS))
Ramos-Cabrer, Pedro (Instituto de Investigación Sanitaria de Santiago (IDIS))

Fecha:	2015
Resumen:	In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from the periphery of the induced cerebral ischemic lesions, using tissue from the contra-lateral hemisphere as a control. Several protein spots (3408) were identified by gel electrophoresis, and 11 showed significant differences in expression between peri-infarct and contra-lateral tissues (at least 3-fold, p < 0. 05). Using COFRADIC, 5412 proteins were identified, with 72 showing a difference in expression. Apart from blood-related proteins (such as serum albumin), both techniques showed that the 70 kDa family of heat shock proteins were highly expressed in the peri-infarct tissue. Further studies by 1D and 2D western blotting and immunohistochemistry revealed that only one member of this family (the inducible form, HSP72 or HSP70i) is specifically expressed by the peri-infarct tissue, while the majority of this family (the constitutive form, HSC70 or HSP70c) is expressed in the whole brain. Our data support that HSP72 is a suitable biomarker of peri-infarct tissue in the ischemic brain.
Ayudas:	Instituto de Salud Carlos III PI11/02161 Instituto de Salud Carlos III CP09/00074 Instituto de Salud Carlos III RETICS/R012/0014 Ministerio de Economía y Competitividad SAF2008-02190
Nota:	Altres ajuts: programa Miguel Servet program i programa Sara Borrell
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Proteòmica ; Isquèmia cerebral ; Proteomic analysis ; Stroke
Publicado en:	Scientific reports, Vol. 5 (2015) , article 12030, ISSN 2045-2322

DOI: 10.1038/srep12030
PMID: 26153530

11 p, 1.4 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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