14-3-3 Proteins Participate in Light Signaling through Association with PHYTOCHROME INTERACTING FACTORs
Adams, Eri (RIKEN Center for Sustainable Resource Science (Japó))
Diaz, Celine (Centre de Recerca en Agrigenòmica)
Hong, Jong-Pil (RIKEN Center for Sustainable Resource Science (Japó))
Shin, Ryoung (RIKEN Center for Sustainable Resource Science (Japó))

Date:	2014
Abstract:	14-3-3 proteins are regulatory proteins found in all eukaryotes and are known to selectively interact with phosphorylated proteins to regulate physiological processes. Through an affinity purification screening, many light-related proteins were recovered as 14-3-3 candidate binding partners. Yeast two-hybrid analysis revealed that the 14-3-3 kappa isoform (14-3-3κ) could bind to PHYTOCHROME INTERACTING FACTOR3 (PIF3) and CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). Further analysis by in vitro pull-down assay confirmed the interaction between 14-3-3κ and PIF3. Interruption of putative phosphorylation sites on the 14-3-3 binding motifs of PIF3 was not suficient to inhibit 14-3-3κ from binding or to disturb nuclear localization of PIF3. It was also indicated that 14-3-3κ could bind to other members of the PIF family, such as PIF1 and PIF6, but not to LONG HYPOCOTYL IN FAR-RED1 (HFR1). 14-3-3 mutants, as well as the PIF3 overexpressor, displayed longer hypocotyls, and a pif3 mutant displayed Shorter hypocotyls than the wild-type in red light, suggesting that 14-3-3 proteins are positive regulators of photomorphogenesis and function antagonistically with PIF3. Consequently, our results indicate that 14-3-3 proteins bind to PIFs and initiate photomorphogenesis in response to a light signal.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	PHYTOCHROME INTERACTING FACTOR (PIF) ; 14-3-3 protein ; Light signaling ; Photomorphogenesis ; Arabidopsis thaliana ; Plant ; Protein-protein interaction
Published in:	International journal of molecular sciences, Vol. 15 Núm. 12 (december 2014) , p. 22801-22814, ISSN 1422-0067

DOI: 10.3390/ijms151222801
PMID: 25501334

14 p, 3.3 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles