Reference values assessment in a Mediterranean population for small dense low-density lipoprotein concentration isolated by an optimized precipitation method
Fernández-Cidón, Bárbara (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Padró-Miquel, Ariadna (Hospital Universitari de Bellvitge)
Alía Ramos, Pedro (Hospital Universitari de Bellvitge)
Castro-Castro, María José (Hospital Universitari de Bellvitge)
Fanlo-Maresma, Marta (Hospital Universitari de Bellvitge)
Dot-Bach, Dolors (Hospital Universitari de Bellvitge)
Valero-Politi, José (Hospital Universitari de Bellvitge)
Pintó, Xavier (Hospital Universitari de Bellvitge)
Candás-Estébanez, Beatriz (Hospital Universitari de Bellvitge)

Data:	2017
Resum:	High serum concentrations of small dense low-density lipoprotein cholesterol (sd-LDL-c) particles are associated with risk of cardiovascular disease (CVD). Their clinical application has been hindered as a consequence of the laborious current method used for their quantification. Optimize a simple and fast precipitation method to isolate sd-LDL particles and establish a reference interval in a Mediterranean population. Forty-five serum samples were collected, and sd-LDL particles were isolated using a modified heparin-Mg 2+ precipitation method. sd-LDL-c concentration was calculated by subtracting high-density lipoprotein cholesterol (HDL-c) from the total cholesterol measured in the supernatant. This method was compared with the reference method (ultracentrifugation). Reference values were estimated according to the Clinical and Laboratory Standards Institute and The International Federation of Clinical Chemistry and Laboratory Medicine recommendations. sd-LDL-c concentration was measured in serums from 79 subjects with no lipid metabolism abnormalities. The Passing-Bablok regression equation is y = 1. 52 (0. 72 to 1. 73) + 0. 07 x (−0. 1 to 0. 13), demonstrating no significant statistical differences between the modified precipitation method and the ultracentrifugation reference method. Similarly, no differences were detected when considering only sd-LDL-c from dyslipidemic patients, since the modifications added to the precipitation method facilitated the proper sedimentation of triglycerides and other lipoproteins. The reference interval for sd-LDL-c concentration estimated in a Mediterranean population was 0. 04-0. 47 mmol/L. An optimization of the heparin-Mg 2+ precipitation method for sd-LDL particle isolation was performed, and reference intervals were established in a Spanish Mediterranean population. Measured values were equivalent to those obtained with the reference method, assuring its clinical application when tested in both normolipidemic and dyslipidemic subjects.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Sd-LDL-c ; Small dense lipoprotein ; Sd-LDL reference values
Publicat a:	Vascular Health and Risk Management, Vol. 13 (june 2017) , p. 201-207, ISSN 1178-2048

DOI: 10.2147/VHRM.S132475
PMID: 28652759

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