Study of the effect of the bacterial and fungal communities present in real wastewatereffluents on the performance of fungal treatments
Badia Fabregat, Marina (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Lucas, Daniel (Universitat de Girona. Institut Català de Recerca de l'Aigua)
Tuomivirta, Tero (Natural Resources Institute Finland (Luke))
Fritze, Hannu (Natural Resources Institute Finland (Luke))
Pennanen, Tania (Natural Resources Institute Finland (Luke))
Rodríguez Mozaz, Sara (Universitat de Girona. Institut Català de Recerca de l'Aigua)
Barceló, Damià (Universitat de Girona. Institut Català de Recerca de l'Aigua)
Caminal i Saperas, Glòria (Institut de Química Avançada de Catalunya)
Vicent i Huguet, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Data:	2017
Resum:	The use of the ligninolytic fungi Trametes versicolor for the degradation of micropollutants has been widely studied. However, few studies have addressed the treatment of real wastewater containing pharmaceutically active compounds (PhAC) under non-sterile conditions. The main drawback of performing such treatments is the difficulty for the inoculated fungus to successfully compete with the other microorganisms growing in the bioreactor. In the present study, several fungal treatments were performed under non-sterile conditions in continuous operational mode with two types of real wastewater effluent, namely, a reverse osmosis concentrate (ROC) from a wastewater treatment plant and a veterinary hospital wastewater (VHW). In all cases, the setup consisted of two parallel reactors: one inoculated with T. versicolor and one non-inoculated, which was used as the control. The main objective of this work was to correlate the operational conditions and traditional monitoring parameters, such as laccase activity, with PhAC removal and the composition of the microbial communities developed inside the bioreactors. For that purpose a variety of biochemical and molecular biology analyses were performed: phospholipid fatty acids analysis (PLFA), quantitative PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE) followed by sequencing. The results show that many indigenous fungi (and not only bacteria, which were the focus of the majority of previously published research) can successfully compete with the inoculated fungi (i. e. , Trichoderma asperellum overtook T. versicolor in the ROC treatment). We also showed that the wastewater origin and the operational conditions had a stronger impact on the diversity of microbial communities developed in the bioreactors than the inoculation or not with T. versicolor.
Ajuts:	Ministerio de Economía y Competitividad CTQ2010-21776-C02 Ministerio de Economía y Competitividad CTM2013-48545-C2 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-291 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-476 Agència de Gestió d'Ajuts Universitaris i de Recerca 2012/BE100857 Ministerio de Educación, Cultura y Deporte AP-2010-4926
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Molecular biology ; Microbial communities ; White-rot fungi ; Bioreactor ; Real effluents ; Pharmaceutically active compounds (PhAC)
Publicat a:	Science of the total environment, Vol. 579, (February 2017) , p. 366-377, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2016.11.088

Postprint 38 p, 3.5 MB

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