Novel regulatory mechanism of establishment genes of conjugative plasmids
Val-Calvo, Jorge (Centro de Biología Molecular Severo Ochoa)
Luque-Ortega, Juan Román (Centro de Investigaciones Biológicas (Madrid))
Crespo, Isidro (ALBA Laboratori de Llum de Sincrotró)
Miguel Arribas, Andrés (Centro de Biología Molecular Severo Ochoa)
Abia, David (Centro de Biología Molecular Severo Ochoa)
Sánchez-Hevia, Dione L. (Centro de Biología Molecular Severo Ochoa)
Serrano, Ester (Centro de Biología Molecular Severo Ochoa)
Gago-Córdoba, César (Centro de Biología Molecular Severo Ochoa)
Ares, Saúl (Universidad Carlos III de Madrid)
Alfonso, Carlos (Centro de Investigaciones Biológicas (Madrid))
Rojo, Fernando (Centro Nacional de Biotecnología (CSIC))
Wu, Ling J. (Newcastle University)
Boer, Roeland (ALBA Laboratori de Llum de Sincrotró)
Meijer, Wilfried J. J. (Centro de Biología Molecular Severo Ochoa)

Data:	2018
Resum:	The principal route for dissemination of antibiotic resistance genes is conjugation by which a conjugative DNA element is transferred from a donor to a recipient cell. Conjugative elements contain genes that are important for their establishment in the new host, for instance by counteracting the host defense mechanisms acting against incoming foreign DNA. Little is known about these establishment genes and how they are regulated. Here, we deciphered the regulation mechanism of possible establishment genes of plasmid p576 from the Gram-positive bacterium Bacillus pumilus. Unlike the ssDNA promoters described for some conjugative plasmids, the four promoters of these p576 genes are repressed by a repressor protein, which we named Reg. Reg also regulates its own expression. After transfer of the DNA, these genes are de-repressed for a period of time until sufficient Reg is synthesized to repress the promoters again. Complementary in vivo and in vitro analyses showed that different operator configurations in the promoter regions of these genes lead to different responses to Reg. Each operator is bound with extreme cooperativity by two Regdimers. The X-ray structure revealed that Reg has a Ribbon-Helix-Helix core and provided important insights into the high cooperativity of DNA recognition.
Ajuts:	Ministerio de Economía y Competitividad BFU2016-75471-C2-1-P Ministerio de Economía y Competitividad BIO2013-41489-P Ministerio de Economía y Competitividad BIO2016-77883-C2-1-P Ministerio de Economía y Competitividad BIO2016-77883-C2-2-P Ministerio de Economía y Competitividad BIO2015-66203-P Ministerio de Economía y Competitividad FIS2016-78313-P
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Nucleic acids research, Vol. 46, Issue 22 (December 2018) , p. 11910-11926, ISSN 1362-4962

DOI: 10.1093/nar/gky996
PMID: 30380104

17 p, 5.8 MB

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