Label-free SPR detection of gluten peptides in urine for non-invasive celiac disease follow-up
Soler Aznar, Maria (Institut Català de Nanociència i Nanotecnologia)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Moreno, María de Lourdes (Universidad de Sevilla)
Cebolla, Angel (Biomedal S.L.)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Data:	2016
Resum:	Motivated by the necessity of new and efficient methods for dietary gluten control of celiac patients, we have developed a simple and highly sensitive SPR biosensor for the detection of gluten peptides in urine. The sensing methodology enables rapid and label-free quantification of the gluten immunogenic peptides (GIP) by using G12 mAb. The overall performance of the biosensor has been in-depth optimized and evaluated in terms of sensitivity, selectivity and reproducibility, reaching a limit of detection of 0. 33ngmL. Besides, the robustness and stability of the methodology permit the continuous use of the biosensor for more than 100 cycles with excellent repeatability. Special efforts have been focused on preventing and minimizing possible interferences coming from urine matrix enabling a direct analysis in this fluid without requiring extraction or purification procedures. Our SPR biosensor has proven to detect and identify gluten consumption by evaluating urine samples from healthy and celiac individuals with different dietary gluten conditions. This novel biosensor methodology represents a novel approach to quantify the digested gluten peptides in human urine with outstanding sensitivity in a rapid and non-invasive manner. Our technique should be considered as a promising opportunity to develop Point-of-Care (POC) devices for an efficient, simple and accurate gluten free diet (GFD) monitoring as well as therapy follow-up of celiac disease patients.
Ajuts:	Ministerio de Economía y Competitividad BES-2010-037704 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-624 Ministerio de Economía y Competitividad SEV-2013-0295
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Matèria:	Surface plasmon resonance ; Gluten immunogenic peptides ; 33-mer gliadin peptide ; Gluten detection ; Celiac disease ; POC device
Publicat a:	Biosensors & bioelectronics, Vol. 79 (May 2016) , p. 158-164, ISSN 1873-4235

DOI: 10.1016/j.bios.2015.11.097

Preprint 15 p, 518.9 KB

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