Assessing the impact on virus transmission and insect vector behavior of a viral mixed infection in melon
Domingo Calap, María Luisa (Centre de Recerca en Agrigenòmica)
Moreno, Ana Beatriz (Centre de Recerca en Agrigenòmica)
Díaz Pendón, Juan Antonio (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora")
Moreno, Aranzazu (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Ciencias Agrarias)
Fereres, Alberto (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Ciencias Agrarias)
Lopez-Moya, Juan Jose (Centre de Recerca en Agrigenòmica)

Date:	2020
Abstract:	Mixed viral infections in plants are common, and can result in synergistic or antagonistic interactions. Except in complex diseases with severe symptoms, mixed infections frequently remain unnoticed, and their impact on insect vector transmission is largely unknown. In this study, we considered mixed infections of two unrelated viruses commonly found in melon plants, the crinivirus cucurbit yellow stunting disorder virus (CYSDV) and the potyvirus watermelon mosaic virus (WMV), and evaluated their vector transmission by whiteflies and aphids, respectively. Their dynamics of accumulation was analyzed until 60 days postinoculation (dpi) in mixed-infected plants, documenting reduced titers of WMV and much higher titers of CYSDV compared with single infections. At 24 dpi, corresponding to the peak of CYSDV accumulation, similar whitefly transmission rates were obtained when comparing either individual or mixed-infected plants as CYSDV sources, although its secondary dissemination was slightly biased toward plants previously infected with WMV, regardless of the source plant. However, at later time points, mixed-infected plants partially recovered from the initially severe symptoms, and CYSDV transmission became significantly higher. Interestingly, aphid transmission rates both at early and late time points were unaltered when WMV was acquired from mixed-infected plants despite its reduced accumulation. This lack of correlation between WMV accumulation and transmission could result from compensatory effects observed in the analysis of the aphid feeding behavior by electrical penetration graphs. Thus, our results showed that mixed-infected plants could provide advantages for both viruses, directly favoring CYSDV dissemination while maintaining WMV transmission.
Grants:	European Commission 657527 Ministerio de Economía y Competitividad AGL2016-75529-R Ministerio de Economía y Competitividad SEV-2015-0533
Note:	Altres ajuts: Generalitat de Catalunya/CERCA Programme
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Virus ; Virology
Published in:	Phytopathology, Vol. 110, issue 1 (Jan. 2020) p. 174-186, ISSN 0031-949X

DOI: 10.1094/PHYTO-04-19-0126-FI

Preprint 43 p, 4.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
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