Involvement of extracellular vesicles in the macrophage-tumor cell communication in head and neck squamous cell carcinoma
Bellmunt, À. M. (Hospital Universitari Vall d'Hebron)
López-Puerto, L. (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Lorente, J. (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Closa Autet, Daniel (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Universitat Autònoma de Barcelona

Data:	2019
Resum:	Background Exosomes are cell-derived vesicles that mediate cellular communication in health and multiple diseases, including cancer. However, its role in head and neck cancer has been poorly defined. Here, we investigated the relevance of exosomes in the signaling between larynx cancer cells and macrophages. Methods Exosomes from THP1 macrophages and BICR18 cells (a larynx squamous cell carcinoma cell line) were purified and their role in the cancer cell migration, macrophage phenotype and immunosuppressive activity was evaluated. The activation of STAT3 signal transduction in macrophages in response to exosomes obtained from cancer cells was also evaluated. Results Macrophages foster the cancer cell migration and this effect is mediated by exosome signaling. On the other hand, exosomes also induce the expression of IL-10 in macrophages and PD-L1 in cancer cells, thus resulting in the promotion of an immunosuppressive environment. Moreover, we observed that the effects induced in cancer cells are mediated by the exosome-depending activation of STAT-3 signal transduction pathway. Conclusions Our study indicates that exosomes released by both macrophages and cancer cells plays a critical role in tumor progression in larynx cancer and might be a potential target for therapeutic intervention in head and neck cancer.
Ajuts:	Instituto de Salud Carlos III PI16/00060
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	B7-H1 Antigen ; Cell Communication ; Cell Movement ; Extracellular Vesicles ; Humans ; Macrophages ; Phenotype ; RNA, Messenger ; Squamous Cell Carcinoma of Head and Neck ; STAT3 Transcription Factor ; THP-1 Cells
Publicat a:	PloS one, Vol. 14 Núm. 11 (2019) , p. e0224710, ISSN 1932-6203

DOI: 10.1371/journal.pone.0224710
PMID: 31697737

13 p, 2.1 MB

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