Expansion of different subpopulations of CD26 −/low T cells in allergic and non-allergic asthmatics
Nieto-Fontarigo, Juan José (Universidade de Santiago de Compostela)
Salgado, F. J. (Universidade de Santiago de Compostela)
San-José, María Esther (Complejo Hospitalario Universitario de Santiago de Compostela)
Cruz Carmona, María Jesús (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Valdés, Luis (Instituto de Investigación Sanitaria de Santiago (IDIS))
Pérez-Díaz, Amparo (Universidade de Santiago de Compostela)
Arias, Pilar (Department of Biochemistry and Molecular Biology. Faculty of Biology-Biological Research Centre (CIBUS). Universidade de Santiago de Compostela)
Nogueira, Montserrat (Department of Biochemistry and Molecular Biology. Faculty of Biology-Biological Research Centre (CIBUS). Universidade de Santiago de Compostela)
González-Barcala, Francisco-Javier (Instituto de Investigación Sanitaria de Santiago (IDIS))
Universitat Autònoma de Barcelona

Data:	2019
Resum:	CD26 displays variable levels between effector (TH ≫ TH > TH > Treg) and naïve/memory (memory > naïve) CD4 T lymphocytes. Besides, IL-6/IL 6R is associated with TH -differentiation and asthma severity. Allergic/atopic asthma (AA) is dominated by TH responses, while TH immunity might either modulate the TH -dependent inflammation in AA or be an important mechanism boosting non-allergic asthma (NAA). Therefore, in this work we have compared the expression of CD26 and CD126 (IL-6Rα) in lymphocytes from different groups of donors: allergic (AA) and non-allergic (NAA) asthma, rhinitis, and healthy subjects. For this purpose, flow cytometry, haematological/biochemical, and in vitro proliferation assays were performed. Our results show a strong CD26-CD126 correlation and an over-representation of CD26 subsets with a highly-differentiated effector phenotype in AA (CD4 CD26 T cells) and NAA (CD4 CD26 γδ-T cells). In addition, we found that circulating levels of CD26 (sCD26) were reduced in both AA and NAA, while loss of CD126 expression on different leukocytes correlated with higher disease severity. Finally, selective inhibition of CD26-mRNA translation led to enhanced T cell proliferation in vitro. These findings support that CD26 down-modulation could play a role in facilitating the expansion of highly-differentiated effector T cell subsets in asthma.
Ajuts:	Ministerio de Economía y Competitividad PI13/02046
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Scientific reports, Vol. 9 Núm. 1 (2019) , p. 7556, ISSN 2045-2322

DOI: 10.1038/s41598-019-43622-8
PMID: 31101830

13 p, 3.7 MB

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