Macrolide resistance and molecular typing of Mycoplasma pneumoniae infections during a 4 year period in Spain
Rivaya, Belén (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Jordana-Lluch, Elena (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Fernandez Rivas, Gema (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Molinos, Sònia (Laboratori Clìnic Metropolitana Nord)
Campos, Roi (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Méndez-Hernández, María (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Matas, Lurdes (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Fecha:	2020
Resumen:	Mycoplasma pneumoniae (MP) causes community-acquired pneumonia affecting mainly children, and tends to produce cyclic outbreaks. The widespread use of macrolides is increasing resistance rates to these antibiotics. Molecular tools can help in diagnosis, typing and resistance detection, leading to better patient management. To assess the MP genotypes and resistance pattern circulating in our area while comparing serological and molecular diagnosis of MP. Molecular and serological diagnosis of MP was performed in 821 samples collected in Badalona (Barcelona, Spain) from 2013 to 2017. Multiple locus variable number tandem repeat analysis (MLVA) and macrolide resistance detection by pyrosequencing were performed in those cases positive by PCR. Presence of respiratory viruses and relevant clinical data were also recorded. MP was detected in 16. 8% of cases by PCR, with an overall agreement with serology of 76%. Eleven different MLVA types were identified, with 4-5-7-2 (50. 1%) and 3-5-6-2 (29. 2%) being the most abundant, with the latter showing a seasonal increase during the study. A total of 8% of the strains harboured a point substitution associated with macrolide resistance, corresponding mainly to an A2063G 23S rRNA mutation and directly related to previous macrolide therapy. Analysis of respiratory viruses showed viral coinfections in most cases. Serological and molecular tools combined could improve MP diagnosis and the analysis of its infection patterns. Macrolide resistance is associated with previous therapy. Given that MP pneumonia usually resolves spontaneously, it should be reconsidered whether antibiotic treatment is suitable for all cases.
Ayudas:	Instituto de Salud Carlos III PI12-02298
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Journal of antimicrobial chemotherapy, Vol. 75 (july 2020) , p. 2752-2759, ISSN 1460-2091

DOI: 10.1093/jac/dkaa256
PMID: 32653897

8 p, 301.2 KB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Artículos > Artículos de investigación
Artículos > Artículos publicados