Identification of type III secretion inhibitors for plant disease management
de Pedro Jové, Roger (Centre de Recerca en Agrigenòmica)
Sebastià, Pau (Centre de Recerca en Agrigenòmica)
Valls, Marc (Centre de Recerca en Agrigenòmica)

Imprint:	New York : Humana, 2021
Description:	10 pàg.
Abstract:	Bacterial plant pathogens are among the most devastating threats to agriculture. To date, there are no effective means to control bacterial plant diseases due to the restrictions in the use of antibiotics in agriculture. A novel strategy under study is the use of chemical compounds that inhibit the expression of key bacterial virulence determinants. The type III secretion system is essential for virulence of many Gram-negative bacteria because it injects into the plant host cells bacterial proteins that interfere with their immune system. Here, we describe the methodology to identify bacterial type III secretion inhibitors, including a series of protocols that combine in planta and in vitro experiments. We use Ralstonia solanacearum as a model because of the number of genetic tools available in this organism and because it causes bacterial wilt, one of the most threatening plant diseases worldwide. The procedures presented can be used to evaluate the effect of different chemical compounds on bacterial growth and virulence.
Rights:	Tots els drets reservats.
Language:	Anglès
Series:	Methods in Molecular Biology ; 2213
Document:	Capítol de llibre ; Versió acceptada per publicar
Subject:	Bacterial plant pathogens ; Type III secretion system ; Ralstonia solanacearum ; Chemical inhibitors ; Plants ; Protocols ; Immunodetection ; In vitro inhibitory test
Published in:	Plant Chemical Genomics: Methods and Protocols, 2021, p. 39-48, ISBN 978-1-0716-0954-5

DOI: 10.1007/978-1-0716-0954-5_4

Postprint 16 p, 633.2 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Books and collections > Book chapters