Shedding plasma membrane vesicles induced by graphene oxide nanoflakes in brain cultured astrocytes
Musto, Mattia (Scuola Internazionale Superiore di Studi Avanzati (Trieste, Itàlia))
Parisse, Pietro (ELETTRA Synchrotron Light Source)
Pachetti, Maria (ELETTRA Synchrotron Light Source)
Memo, Christian (Scuola Internazionale Superiore di Studi Avanzati (Trieste, Itàlia))
Di Mauro, Giuseppe. (Scuola Internazionale Superiore di Studi Avanzati)
Ballesteros, Belén (Institut Català de Nanociència i Nanotecnologia)
Lozano, Neus (Institut Català de Nanociència i Nanotecnologia)
Kostarelos, Kostas (Institut Català de Nanociència i Nanotecnologia)
Casalis, Loredana (ELETTRA Synchrotron Light Source)
Ballerini, Laura (Scuola Internazionale Superiore di Studi Avanzati (Trieste, Itàlia))

Data:	2021
Resum:	Microvesicles (MVs) generated and released by astrocytes, the brain prevalent cells, crucially contribute to intercellular communication, representing key vectorized systems able to spread and actively transfer signaling molecules from astrocytes to neurons, ultimately modulating target cell functions. The increasing clinical relevance of these signaling systems requires a deeper understanding of MV features, currently limited by both their nanoscale dimensions and the low rate of their constituent release. Hence, to investigate the features of such glial signals, nanotechnology-based approaches and the applications of unconventional, cost-effective tools in generating MVs are needed. Here, small graphene oxide (s-GO) nanoflakes are used to boost MVs shedding from astrocytes in cultures and s-GO generated MVs are compared with those generated by a natural stimulant, namely ATP, by atomic force microscopy, light scattering, attenuated total reflection-fourier transform infra-red and ultraviolet resonance Raman spectroscopy. We also report the ability of both types of MVs, upon acute and transient exposure of patch clamped cultured neurons, to modulate basal synaptic transmission, inducing a stable increase in synaptic activity accompanied by changes in neuronal plasma membrane elastic features.
Ajuts:	European Commission 785219 European Commission 881603
Nota:	Altres ajuts: MM, PP and LC acknowledge CERIC-ERIC proposal grant n. 20167063 for the IR measurements, performed at the SISSI-Bio beamline of Elettra Sincrotrone Trieste. PP and LC acknowledge also the European Regional Development Fund and Interreg V-A Italia-Austria 2014-2020 project EXOTHERA (ITAT1036).
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Graphene oxide ; Extracellular vesicles ; Atomic force microscopy and spectroscopy ; FTIR-ATR and UVRR spectroscopy ; Synaptic activity ; Cortical neuronal cultures
Publicat a:	Carbon, Vol. 176 (May 2021) , p. 458-469, ISSN 0008-6223

DOI: 10.1016/j.carbon.2021.01.142

14 p, 2.4 MB

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