Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits
Gardela Santacruz, Jaume (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Jauregi-Miguel, Amaia (Linköping University. Division of Molecular Medicine and Virology)
Martinez, Cristina A.. (Linköping University. Department of Biomedical and Clinical Sciences)
Rodriguez-Martinez, Heriberto (Linköping University. Department of Biomedical and Clinical Sciences)
López Béjar, Manel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Álvarez-Rodríguez, Manuel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Fecha:	2020
Resumen:	Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium interactions, ovulation, and pre-implantation effects (nerve growth factor, NGF; α/β hydrolase domain-containing protein 2, ABHD2; C-terminal tensin-like protein, CTEN or TNS4; and versican, VCAN) in the period 10-72 h post-mating. In Experiment 1, does (n = 9) were treated with gonadotropin-releasing hormone (GnRH) (control), GnRH-stimulated, and vaginally infused with sperm-free seminal plasma (SP-AI), or GnRH-stimulated and naturally mated (NM). In Experiment 2, does (n = 15) were GnRH-stimulated and naturally mated. Samples were retrieved from the internal reproductive tracts (cervix-to-infundibulum) 20 h post-treatment (Experiment 1) or sequentially collected at 10, 24, 36, 68, or 72 h post-mating (Experiment 2, 3 does/period). All samples were processed for gene expression analysis by quantitative PCR. Data showed an upregulation of endometrial CTEN and NGF by NM, but not by SP-AI. The findings suggest that the NGF gene affects the reproductive tract of the doe during ovulation and beyond, influencing the maternal environment during early embryonic development.
Ayudas:	Ministerio de Ciencia e Innovación IJDC-2015-24380
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Gene expression ; Qpcr ; Female reproductive tract ; Induced ovulator
Publicado en:	Genes, Vol. 11 (july 2020) , ISSN 2073-4425

DOI: 10.3390/genes11070758
PMID: 32645906

16 p, 1.2 MB

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