Activation of Eosinophils and Mast Cells in Functional Dyspepsia : an Ultrastructural Evaluation
Vanheel, Hanne (KU Leuven)
Vicario Perez, Maria (Hospital Universitari Vall d'Hebron)
Boesmans, Werend (Maastricht University Medical Center)
Vanuytsel, Tim (KU Leuven)
Salvo-Romero, Eloisa (Hospital Universitari Vall d'Hebron)
Tack, Jan (KU Leuven. Translational Research Center for Gastrointestinal Disorders)
Farré, Ricard (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Universitat Autònoma de Barcelona

Data:	2018
Resum:	We recently identified mucosal mast cell and eosinophil hyperplasia in association with a duodenal impaired barrier function in functional dyspepsia (FD). We aimed to further describe the implication of these immune cells by assessing their activation state at the ultrastructural level and by evaluating the association between impaired epithelial integrity and immune activation. Duodenal biopsies were obtained from 24 FD patients and 37 healthy controls. The ultrastructure of mast cells and eosinophils was analyzed by transmission electron microscopy. Transepithelial electrical resistance and paracellular permeability were measured to evaluate epithelial barrier function. The type of degranulation in eosinophils and mast cells was piecemeal. Eosinophils displayed higher degree of degranulation in FD patients than in controls (p < 0. 0001). Quantification revealed a decreased granular density in eosinophils of FD patients (p < 0. 0001). The degree of degranulation in mast cells was similar in both groups. However, a more heterogeneous profile was found in the FD group (p < 0. 0001). No association between epithelial integrity and the number and activation state of mucosal eosinophils and mast cells was found. We demonstrated ultrastructural changes in degranulation state of eosinophils and mast cells, suggesting that eosinophil and mast cell activation play a role in the pathophysiology of FD.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Scientific reports, Vol. 8 (march 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-018-23620-y
PMID: 29599471

9 p, 1.6 MB

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