Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue
D'Antin, Justin Christopher (Centre d'Oftalmologia Barraquer (Catalunya))
Barraquer, Rafael I. (Rafael Ignasi)
Tresserra, Francisco (Institut Universitari Dexeus. Department de Patologia)
Michael, Ralph (Centre d'Oftalmologia Barraquer (Catalunya))
Universitat Autònoma de Barcelona

Data:	2018
Resum:	In order to determine whether posterior capsule opacification after cataract surgery, could be delayed or inhibited through the application of hydrogen peroxide (HO) or distilled water (HOd),we extracted lens capsules from 25 human donor eye globes. Samples were treated for 5 min with either 30 mM HO or HOd or used as controls, and cultured for one month, during which dark field and tilt illumination photos were taken. These were used to observe and quantify, time until cellular growth and confluence on the posterior capsule. After culture, histological sections were stained for H&E, α-SMA, Ki-67 and vimentin and evaluated. We prevented cellular growth in 50% of HOd and 58% HO of treated samples. The overall prevention of cell growth compared to cultured controls was significant for both treatments while there was no significant difference between them. In the cases where cellular growth was not prevented, both treatments significantly delay cellular growth. Until day 28 none of the treated samples of either type that had shown growth reached total confluence. All cultured controls reached total confluence before treated samples (median = day 11. 5). Also, histologically, there was a clear morphological difference between cultured controls and treated samples.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Scientific reports, Vol. 8 (august 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-018-31178-y
PMID: 30143742

10 p, 2.1 MB

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