Carbapenemase-producing enterobacteriaceae recovered from a Spanish river ecosystem
Piedra-Carrasco, Núria (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Fàbrega-Santamaria, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Calero-Cáceres, William (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Cornejo-Sánchez, Thais (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Brown-Jaque, Maryury (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Mir-Cros, Alba (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Muniesa, Maite (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
González-López, Juanjo 1975- (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Data:	2017
Resum:	The increasing resistance to carbapenems is an alarming threat in the fight against multiresistant bacteria. The dissemination properties of antimicrobial resistance genes are supported by their detection in a diverse population of bacteria, including strains isolated from the environment. The objective of this study was to investigate the presence of carbapenemase-producing Enterobacteriaceae (CPE) collected from a river ecosystem in the Barcelona metropolitan area (Spain). Identification of β-lactamases and other resistance determinants was determined as was the antimicrobial susceptibility profile. Moreover, screening of virulence factors, plasmid addiction systems, plasmid partition systems and replicon typing was performed. The results identified 8 isolates belonging to different species (Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Klebsiella oxytoca, Raoultella ornithinolytica). The most prevalent enzyme was KPC-2 (n = 6), followed by VIM-1 (n = 2) and IMI-2 (n = 1), whereas no OXA-48-type was detected. In addition, one strain was positive for both KPC-2 and VIM-1 enzymes. All the carbapenemase-encoding plasmids carried at least one plasmid addiction or partition system, being vagCD and parAB the most frequently detected, respectively. E. coli and K. pneumoniae isolates carried a low number of virulence-associated factors and none of the detected clones has previously been identified in the clinical setting. These findings support the high dissemination potential of the carbapanemase-encoding genes and reinforce the idea that the environment is another reservoir that may play an important role in the capture, selection and dissemination of carbapenem resistance genes.
Ajuts:	Ministerio de Economía y Competitividad RD12/0015/0003 Ministerio de Economía y Competitividad PI15/00604
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan aquestes es distribueixin sota la mateixa llicència que regula l'obra original i es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 12 (april 2017) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0175246
PMID: 28380016

11 p, 1.3 MB

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