First insights into the pleiotropic role of vrf (yedF), a newly characterized gene of Salmonella Typhimurium
Ballesté-Delpierre, Clara (Barcelona Institute for Global Health (ISGlobal))
Fernandez-Orth, Dietmar (Centre de Recerca en Epidemiologia Ambiental)
Ferrer-Navarro, Mario (Barcelona Institute for Global Health (ISGlobal))
Díaz-Peña, Ramón (Parc Científic de Barcelona)
Odena-Caballol, Antonia (Parc Científic de Barcelona)
Oliveira, Eliandre (Parc Científic de Barcelona)
Fàbrega-Santamaria, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Vila Estapé, Jordi (Barcelona Institute for Global Health (ISGlobal))
Universitat Autònoma de Barcelona

Data:	2017
Resum:	Salmonella possesses virulence determinants that allow replication under extreme conditions and invasion of host cells, causing disease. Here, we examined four putative genes predicted to encode membrane proteins (ydiY, ybdJ, STM1441 and ynaJ) and a putative transcriptional factor (yedF). These genes were identified in a previous study of a S. Typhimurium clinical isolate and its multidrug-resistant counterpart. For STM1441 and yedF a reduced ability to interact with HeLa cells was observed in the knock-out mutants, but an increase in this ability was absent when these genes were overexpressed, except for yedF which phenotype was rescued when yedF was restored. In the absence of yedF, decreased expression was seen for: i) virulence-related genes involved in motility, chemotaxis, attachment and survival inside the host cell; ii) global regulators of the invasion process (hilA, hilC and hilD); and iii) factors involved in LPS biosynthesis. In contrast, an increased expression was observed for anaerobic metabolism genes. We propose yedF is involved in the regulation of Salmonella pathogenesis and contributes to the activation of the virulence machinery. Moreover, we propose that, when oxygen is available, yedF contributes sustained repression of the anaerobic pathway. Therefore, we recommend this gene be named vrf, for virulence-related factor.
Ajuts:	Ministerio de Economía y Competitividad RD12/0015
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Scientific reports, Vol. 7 (november 2017) , ISSN 2045-2322

DOI: 10.1038/s41598-017-15369-7
PMID: 29127378

11 p, 1.8 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats