Activation of Immune and Defense Responses in the Intestinal Mucosa by Outer Membrane Vesicles of Commensal and Probiotic Escherichia coli Strains
Fábrega, María José (Universitat de Barcelona. Departament de Bioquímica i Biomedicina Molecular)
Aguilera, Laura (Universitat de Barcelona. Departament de Bioquímica i Biomedicina Molecular)
Giménez, Rosa (Universitat de Barcelona. Departament de Bioquímica i Biomedicina Molecular)
Varela, Encarna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Alexandra Cañas, María (Universitat de Barcelona. Departament de Bioquímica i Biomedicina Molecular)
Antolín, María (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Badía, Josefa (Universitat de Barcelona. Departament de Bioquímica i Biomedicina Molecular)
Baldomà, Laura (Universitat de Barcelona. Departament de Bioquímica i Biomedicina Molecular)
Universitat Autònoma de Barcelona

Data:	2016
Resum:	The influence of microbiota in human health is well-known. Imbalances in microbiome structure have been linked to several diseases. Modulation of microbiota composition through probiotic therapy is an attempt to harness the beneficial effects of commensal microbiota. Although, there is wide knowledge of the responses induced by gut microbiota, the microbial factors that mediate these effects are not well-known. Gram-negative bacteria release outer membrane vesicles (OMVs) as a secretion mechanism of microbial factors, which have an important role in intercellular communication. Here, we investigated whether OMVs from the probiotic Escherichia coli strain Nissle 1917 (EcN) or the commensal E. coli strain ECOR12 trigger immune responses in various cellular models: (i) peripheral blood mononuclear cells (PBMCs) as a model of intestinal barrier disruption, (ii) apical stimulation of Caco-2/PMBCs co-culture as a model of intact intestinal mucosa, and (iii) colonic mucosa explants as an ex vivo model. Stimulations with bacterial lysates were also performed. Whereas, both OMVs and lysates activated expression and secretion of several cytokines and chemokines in PBMCs, only OMVs induced basolateral secretion and mRNA upregulation of these mediators in the co-culture model. We provide evidence that OMVs are internalized in polarized Caco-2 cells. The activated epithelial cells elicit a response in the underlying immunocompetent cells. The OMVs effects were corroborated in the ex vivo model. This experimental study shows that OMVs are an effective strategy used by beneficial gut bacteria to communicate with and modulate host responses, activating signaling events through the intestinal epithelial barrier.
Ajuts:	Agencia Estatal de Investigación PI12/00263 Ministerio de Economía y Competitividad PI12/00263
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Nissle 1917 ; Probiotics ; Gut microbes ; Membrane vesicles ; Microbiota-host crosstalk ; Intestinal mucosa ; Caco-2/PBMC co-culture
Publicat a:	Frontiers in microbiology, Vol. 7 (may 2016) , ISSN 1664-302X

DOI: 10.3389/fmicb.2016.00705
PMID: 27242727

14 p, 1.4 MB

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