Transversal gene expression panel to evaluate intestinal health in broiler chickens in different challenging conditions
Criado Mesas, Lourdes (Centre de Recerca en Agrigenòmica)
Abdelli, Nedra (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Noce, Antonia (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Farré, Mercè (Universitat Autònoma de Barcelona. Departament de Matemàtiques)
Pérez Hernández, José Francisco (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Solà Oriol, D. (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Martin Venegas, R. (Universitat de Barcelona)
Forouzandeh, Asal (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
González Solé, Francesc (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Folch, Josep M. (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Date:	2021
Abstract:	There is a high interest on gut health in poultry with special focus on consequences of the intestinal diseases, such as coccidiosis and C. perfringens -induced necrotic enteritis (NE). We developed a custom gene expression panel, which could provide a snapshot of gene expression variation under challenging conditions. Ileum gene expression studies were performed through high throughput reverse transcription quantitative real-time polymerase chain reaction. A deep review on the bibliography was done and genes related to intestinal health were selected for barrier function, immune response, oxidation, digestive hormones, nutrient transport, and metabolism. The panel was firstly tested by using a nutritional/ Clostridium perfringens model of intestinal barrier failure (induced using commercial reused litter and wheat-based diets without exogenous supplementation of enzymes) and the consistency of results was evaluated by another experiment under a coccidiosis challenge (orally gavaged with a commercial coccidiosis vaccine, 90× vaccine dose). Growth traits and intestinal morphological analysis were performed to check the gut barrier failure occurrence. Results of ileum gene expression showed a higher expression in genes involved in barrier function and nutrient transport in chickens raised in healthy conditions, while genes involved in immune response presented higher expression in C. perfringens -challenged birds. On the other hand, the Eimeria challenge also altered the expression of genes related to barrier function and metabolism, and increased the expression of genes related to immune response and oxidative stress. The panel developed in the current study gives us an overview of genes and pathways involved in broiler response to pathogen challenge. It also allows us to deep into the study of differences in gene expression pattern and magnitude of responses under either a coccidial vaccine or a NE.
Grants:	Ministerio de Economía y Competitividad BES-2015-075403
Note:	Altres ajuts: Gestió d'Ajuts Universitaris i de Recerca de la Generalitat de Catalunya (PhD Grant 2017FI; NA)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Genetics ; Molecular biology
Published in:	Scientific reports, Vol. 11 (March 2021) , art. 6315, ISSN 2045-2322

DOI: 10.1038/s41598-021-85872-5
PMID: 33737699

14 p, 2.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles