Symptomatic and Asymptomatic Protist Infections in Hospital Inpatients in Southwestern China
Zhang, Shun-Xian (Chinese Center for Tropical Diseases Research)
Carmena, David (National Centre for Microbiology, Health Institute Carlos III)
Ballesteros, Cristina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Yang, Chun-Li (Hospital of People's Liberation Army of China)
Chen, Jia-Xu (Shanghai Jiao Tong University School of Medicine)
Chu, Yan-Hong (Shanghai Jiao Tong University School of Medicine)
Yu, Ying-Fang (Shanghai Jiao Tong University School of Medicine)
Wu, Xiu-Ping (Shanghai Jiao Tong University School of Medicine)
Tian, Li-Guang (Shanghai Jiao Tong University School of Medicine)
Serrano Ferron, Emmanuel (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2021
Resum:	Cryptosporidium spp. , Entamoeba histolytica, Giardia duodenalis, and Blastocystis sp. infections have been frequently reported as etiological agents for gastroenteritis, but also as common gut inhabitants in apparently healthy individuals. Between July 2016 and March 2017, stool samples (n = 507) were collected from randomly selected individuals (male/female ratio: 1. 1, age range: 38-63 years) from two sentinel hospitals in Tengchong City Yunnan Province, China. Molecular (PCR and Sanger sequencing) methods were used to detect and genotype the investigated protist species. Carriage/infection rates were: Blastocystis sp. 9. 5% (95% CI: 7. 1-12. 4%), G. duodenalis 2. 2% (95% CI: 1. 1-3. 8%); and E. histolytica 2. 0% (95% CI: 0. 9-3. 6%). Cryptosporidium spp. was not detected at all. Overall, 12. 4% (95% CI: 9. 7-15. 6) of the participants harbored at least one enteric protist species. The most common coinfection was E. histolytica and Blastocystis sp. (1. 0%; 95% CI: 0. 3-2. 2). Sequence analyses revealed that 90. 9% (10/11) of the genotyped G. duodenalis isolates corresponded to the sub-assemblage AI. The remaining sequence (9. 1%, 1/11) was identified as sub-assemblage BIV. Five different Blastocystis subtypes, including ST3 (43. 7%, 21/48), ST1 (27. 1%, 13/48), ST7 (18. 8%, 9/48), ST4 (8. 3%, 4/48), and ST2 (2. 1%, 1/48) were identified. Statistical analyses confirmed that (i) the co-occurrence of protist infections was purely random, (ii) no associations were observed among the four protist species found, and (iii) neither their presence, individually or jointly, nor the patient's age was predictors for developing clinical symptoms associated with these infections. Overall, these protist mono- or coinfections are asymptomatic and do not follow any pattern.
Ajuts:	Ministerio de Economía y Competitividad RYC-2016-21120
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Coinfection ; Enteric protists ; China ; Giardia duodenalis ; Entamoeba histolytica ; Cryptosporidium ; Blastocystis sp ; Genotyping ; Molecular diversity
Publicat a:	Pathogens, Vol. 10 (may 2021) , ISSN 2076-0817

DOI: 10.3390/pathogens10060684
PMID: 34072858

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