One-step and real-time detection of microRNA-21 in human samples for lung cancer biosensing diagnosis
Calvo Lozano, Olalla (Institut Català de Nanociència i Nanotecnologia)
García Aparicio, Pablo (Institut Català de Nanociència i Nanotecnologia)
Raduly, Lajos-Zsolt (Iuliu Hatieganu University of Medicine and Pharmacy)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Berindan Neagoe, Ioana (Iuliu Hatieganu University of Medicine and Pharmacy)
Ferracin, Manuela (Università di Bologna. Dipartimento di Scienze Mediche e Chirurgiche)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Data:	2022
Resum:	The rapid diagnosis of cancer, especially in its early stages, is crucial for on-time medical treatment and for increasing the patient survival rate. Lung cancer shows the highest mortality rate and the lowest 5-year survival rate due to the late diagnosis in advanced cancer stages. Providing rapid and reliable diagnostic tools is a top priority to address the problem of a delayed cancer diagnosis. We introduce a nanophotonic biosensor for the direct and real-time detection in human plasma of the microRNA-21-5p biomarker related to lung cancer. The biosensor employs a silicon photonic bimodal interferometric waveguide that provides a highly sensitive detection in a label-free format. We demonstrate a very competitive detectability for direct microRNA-21-5p biomarker assays in human plasma samples (estimated LOD: 25 pM). The diagnostic capability of our biosensor was validated by analyzing 40 clinical samples from healthy individuals and lung cancer patients, previously analyzed by reverse-transcription quantitative polymerase chain reaction (qRT-PCR). We could successfully identify and quantify the levels of microRNA in a one-step assay, without the need for DNA extraction or amplification steps. The study confirmed the significance of implementing this biosensor technique compared to the benchmarking molecular analysis and showed excellent agreement with previous results employing the traditional qRT-PCR. This work opens new possibilities for the true implementation of point-of-care biosensors that enable fast, simple, and efficient early diagnosis of cancer diseases.
Ajuts:	Agencia Estatal de Investigación PID2019-105132RB-I00 Agencia Estatal de Investigación PCI2019-111890-2 Agencia Estatal de Investigación TEC2016-78515-R Agencia Estatal de Investigación SEV-2017-0706 Agencia Estatal de Investigación BES-2017-080527
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biotechnology ; Cancer ; Genetics ; Liquid chromatography ; Sensors
Publicat a:	Analytical chemistry, Vol. 94, issue 42 (Oct. 2022) , p. 14659-14665, ISSN 1520-6882

DOI: 10.1021/acs.analchem.2c02895
PMID: 36219565

7 p, 2.4 MB

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