The mRNA expression of the three major described cold-inducible proteins, including CIRBP, differs in the bovine endometrium and ampulla during the estrous cycle
Gardela Santacruz, Jaume (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Ruiz Conca, Mateo (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Olvera-Maneu, Sergi (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
López Béjar, Manel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Álvarez-Rodríguez, Manuel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Date:	2022
Abstract:	The cold-inducible proteins (CIPs) are essential for post-transcriptional gene regulation playing diverse tissue-specific roles in maintaining normal cellular function and morphogenesis. The potential implications of CIPs in reproductive events raise questions about their role in the physiology of the bovine reproductive tract. However, the expression changes of CIPs during the bovine estrous cycle have not been studied so far. Here, we hypothesized that the bovine estrous cycle could affect the mRNA expression of the CIPs and other candidate transcripts in the reproductive tract. This study aimed to examine estrous cycle-dependent mRNA expression patterns in the bovine endometrium and ampulla of three of the major described CIPs (CIRBP, RBM3, SRSF5), a set of inflammatory cytokines (IL-10, IL-18, IL-1β), and other candidate genes (IL-10RA, IL-10RB, BCL2, NLRP3, STAT1, STAT3, STAT5A, STAT6). Endometrial and ampullar tissues were assessed by RT-qPCR. Additionally, the mRNA expression levels were correlated among them and with follicular progesterone and estradiol concentrations. The transcript levels of CIPs increased in the endometrium during stage III (Days 11-17) compared to stage I (Days 1-4) and IV (Days 18-20). In the ampulla, the mRNA expression of CIRBP increased during the late luteal phase (stage III), but no differences in the expression of other CIPs were observed. This study expands the current knowledge regarding mRNA expression in the endometrium and oviductal ampulla of cycling heifers, focusing mainly on the CIPs. A better understanding of the mechanisms within the uterus and oviduct during the estrous cycle is crucial to improving the fertility rate.
Grants:	Ministerio de Educación, Cultura y Deporte EST19 /00848 Agència de Gestió d'Ajuts Universitaris i de Recerca 2018FI_00236 Ministerio de Educación, Cultura y Deporte FPU15/06029 Ministerio de Ciencia e Innovación PID2019-108320RJ-I00 Ministerio de Ciencia e Innovación IJCI-2015-24380
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Research in veterinary science, Vol. 152 (20 2022) , p. 181-189, ISSN 1532-2661

DOI: 10.1016/j.rvsc.2022.08.006
PMID: 35987103

9 p, 2.0 MB

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