Coating bioactive microcapsules with tannic acid enhances the phenotype of the encapsulated pluripotent stem cells
Choi, Daheui (Mayo Clinic, Department of Physiology and Biomedical Engineering)
Gwon, Kihak (Mayo Clinic. Department of Physiology and Biomedical Engineering)
Hong, Hye Jin (Mayo Clinic. Department of Physiology and Biomedical Engineering)
Baskaran, Harihara (Case Western Reserve University. Department of Chemical and Biomolecular Engineering)
Calvo Lozano, Olalla (Institut Català de Nanociència i Nanotecnologia)
Gonzalez-Suarez, Alan M. (Mayo Clinic. Department of Physiology and Biomedical Engineering)
Park, Kyungtae (Yonsei University. Department of Chemical and Biomolecular Engineering)
De Hoyos-Vega, Jose M. (Mayo Clinic. Department of Physiology and Biomedical Engineering)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)
Hong, Jinkee (Yonsei University. Department of Chemical and Biomolecular Engineerin)
Stybayeva, Gulnaz (Mayo Clinic. Department of Physiology and Biomedical Engineering)
Revzin, Alexander (Mayo Clinic. Department of Physiology and Biomedical Engineering)

Títol variant:	Ultrathin coating of bioactive microcapsules enhances phenotype of encapsulated pluripotent stem cells
Data:	2022
Resum:	Human pluripotent stem cells (hPSCs) may be differentiated into any adult cell type and therefore hold incredible promise for cell therapeutics and disease modeling. There is increasing interest in three-dimensional (3D) hPSC culture because of improved differentiation outcomes and potential for scale up. Our team has recently described bioactive heparin (Hep)-containing core-shell microcapsules that promote rapid aggregation of stem cells into spheroids and may also be loaded with growth factors for the local and sustained delivery to the encapsulated cells. In this study, we explored the possibility of further modulating bioactivity of microcapsules through the use of an ultrathin coating composed of tannic acid (TA). Deposition of the TA film onto model substrates functionalized with Hep and poly(ethylene glycol) was characterized by ellipsometry and atomic force microscopy. Furthermore, the presence of the TA coating was observed to increase the amount of basic fibroblast growth factor (bFGF) incorporation by up to twofold and to extend its release from 5 to 7 days. Most significantly, TA-microcapsules loaded with bFGF induced higher levels of pluripotency expression compared to uncoated microcapsules containing bFGF. Engineered microcapsules described here represent a new stem cell culture approach that enables 3D cultivation and relies on local delivery of inductive cues.
Nota:	El títol alternatiu correspon al preprint de l'article publicat.
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Matèria:	Microfluidics ; Heparin-based microcapsules ; Tannic acid ; Nanofilm ; Controlled growth factor release ; 3D stem cell cultures
Publicat a:	ACS applied materials & interfaces, Vol. 14, issue 23 (June 2022) , p. 27274-27286, ISSN 1944-8252

DOI: 10.1021/acsami.2c06783
PMID: 35658394

Preprint 48 p, 1.4 MB

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