Cervical Fluids Are a Source of Protein Biomarkers for Early, Non-Invasive Endometrial Cancer Diagnosis
Martinez-Garcia, Elena (Luxembourg Institute of Health)
Coll de la Rubia, Eva (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Lesur, Antoine (Luxembourg Institute of Health)
Dittmar, Gunnar (University of Luxembourge)
Gil-Moreno, Antonio 1965- (Hospital Universitari Vall d'Hebron)
Cabrera Díaz, Silvia (Hospital Universitari Vall d'Hebron)
Colás Ortega, Eva (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Data:	2023
Resum:	Abnormal uterine bleeding (AUB) is the main symptom of endometrial cancer (EC), but it is highly nonspecific. This represents a huge burden for women's health, since all women presenting with bleeding will undergo sequential invasive tests, avoidable in 90-95% of those women who do not have EC. This study aimed to evaluate the potential of cervical samples collected with five different devices as a source of EC diagnostic protein biomarkers. Samples collected with a Rovers Cervex Brush ® and the HC2 DNA collection device, Digene, were the most suitable for EC proteomic studies. A clinical retrospective study assessing the expression of 52 EC-related proteins in 41 patients (22 EC; 19 non-EC), by targeted proteomics, identified SERPINH1, VIM, TAGLN, PPIA, CSE1L, and CTNNB1 as potential EC protein biomarkers in cervical fluids (AUC > 0. 8). This study opens an avenue for developing non-invasive protein-based EC diagnostic tests, which will improve the standard of care for gynecological patients. Background: Abnormal uterine bleeding is the main symptom of endometrial cancer (EC), but it is highly nonspecific. This represents a huge burden for women's health since all women presenting with bleeding will undergo sequential invasive tests, which are avoidable for 90-95% of those women who do not have EC. Methods: This study aimed to evaluate the potential of cervical samples collected with five different devices as a source of protein biomarkers to diagnose EC. We evaluated the protein quantity and the proteome composition of five cervical sampling methods. Results: Samples collected with a Rovers Cervex Brush ® and the HC2 DNA collection device, Digene, were the most suitable samples for EC proteomic studies. Most proteins found in uterine fluids were also detected in both cervical samples. We then conducted a clinical retrospective study to assess the expression of 52 EC-related proteins in 41 patients (22 EC; 19 non-EC), using targeted proteomics. We identified SERPINH1, VIM, TAGLN, PPIA, CSE1L, and CTNNB1 as potential protein biomarkers to discriminate between EC and symptomatic non-EC women with abnormal uterine bleeding in cervical fluids (AUC > 0. 8). Conclusions: This study opens an avenue for developing non-invasive protein-based EC diagnostic tests, which will improve the standard of care for gynecological patients.
Ajuts:	Instituto de Salud Carlos III PI17/02155 Instituto de Salud Carlos III PI20/00644 Instituto de Salud Carlos III IFI19/00029 Ministerio de Economía y Competitividad CB16/12/00328 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021SGR1157
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Carcinoma of the endometrium ; Endometrial cancer ; Uterine cancer ; Biomarker ; Diagnosis ; Gynecology ; Endometrial sampling ; Proteomics ; Cervical sample ; Protein ; Non-invasive
Publicat a:	Cancers, Vol. 15 (january 2023) , ISSN 2072-6694

DOI: 10.3390/cancers15030911
PMID: 36765869

17 p, 2.5 MB

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