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Página principal > Artículos > Artículos publicados > Recombinant Proteins for Assembling as Nano- and Micro-Scale Materials for Drug Delivery : |
Fecha: | 2023 |
Resumen: | By following simple protein engineering steps, recombinant proteins with promising applications in the field of drug delivery can be assembled in the form of functional materials of increasing complexity, either as nanoparticles or nanoparticle-leaking secretory microparticles. Among the suitable strategies for protein assembly, the use of histidine-rich tags in combination with coordinating divalent cations allows the construction of both categories of material out of pure polypeptide samples. Such molecular crosslinking results in chemically homogeneous protein particles with a defined composition, a fact that offers soft regulatory routes towards clinical applications for nanostructured protein-only drugs or for protein-based drug vehicles. Successes in the fabrication and final performance of these materials are expected, irrespective of the protein source. However, this fact has not yet been fully explored and confirmed. By taking the antigenic RBD domain of the SARS-CoV-2 spike glycoprotein as a model building block, we investigated the production of nanoparticles and secretory microparticles out of the versions of recombinant RBD produced by bacteria (Escherichia coli), insect cells (Sf9), and two different mammalian cell lines (namely HEK 293F and Expi293F). Although both functional nanoparticles and secretory microparticles were effectively generated in all cases, the technological and biological idiosyncrasy of each type of cell factory impacted the biophysical properties of the products. Therefore, the selection of a protein biofabrication platform is not irrelevant but instead is a significant factor in the upstream pipeline of protein assembly into supramolecular, complex, and functional materials. |
Ayudas: | Agència de Gestió d'Ajuts Universitaris i de Recerca 2020/PANDE-00003 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00092 Agencia Estatal de Investigación PID2019-105416RB-I00 Agencia Estatal de Investigación PID2019-107298RB-C22 Agencia Estatal de Investigación PID2020-116174RB-I00 |
Nota: | Altres ajuts: CIBER - Consorcio Centro de Investigación Biomédica en Red CB06/01/0014; María Zambrano postdoctoral researcher contract (677904) |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió publicada |
Materia: | Recombinant proteins ; Protein materials ; Cell factory ; Nanoparticles ; Microparticles ; Building blocks ; Biomimetics ; Protein secretion |
Publicado en: | Pharmaceutics, Vol. 15, Issue 4 (April 2023) , art. 1197, ISSN 1999-4923 |
Obra relacionada: | Corchero Nieto, José Luis; de Pinho Favaro, Marianna T.; Márquez-Martínez, Mercè; Lascorz Lozano, Jara A.; Martínez-Torró, Carlos; Sanchez, Julieta M.; López-Laguna, Hèctor; de Souza Ferreira, Luís Carlos; Vázquez Gómez, Esther; Ferrer-Miralles, Neus; Villaverde Corrales, Antonio; Parladé Molist, Eloi, 2023, "Raw data to support the manuscript with the following scope: Host comparative analysis of protein materials sources", CORA.Repositori de Dades de Recerca, V1 https://doi.org/10.34810/data685 |
16 p, 2.5 MB |