An LTR retrotransposon in the promoter of a PsMYB10.2 gene associated with the regulation of fruit flesh color in Japanese plum
Fiol, Arnau (Centre de Recerca en Agrigenòmica)
García Carbonell, Sergio (Centre de Recerca en Agrigenòmica)
Dujak, Christian (Centre de Recerca en Agrigenòmica)
Pacheco, Igor (Universidad de Chile. Instituto de Nutrición y Tecnología de Alimentos)
Infante, Rodrigo (Universidad de Chile. Departamento de Producción Agrícola)
Aranzana, Maria José (Centre de Recerca en Agrigenòmica)

Date:	2022
Abstract:	Japanese plums exhibit wide diversity of fruit coloration. The red to black hues are caused by the accumulation of anthocyanins, while their absence results in yellow, orange or green fruits. In Prunus, MYB10 genes are determinants for anthocyanin accumulation. In peach, QTLs for red plant organ traits map in an LG3 region with three MYB10 copies (PpMYB10. 1, PpMYB10. 2 and PpMYB10. 3). In Japanese plum the gene copy number in this region differs with respect to peach: there are at least three copies of PsMYB10. 1, with the expression of one of them (PsMYB10. 1a) correlating with fruit skin color. The objective of this study was to determine a possible role of LG3-PsMYB10 genes in the natural variability of the flesh color trait and to develop a molecular marker for marker-assisted selection (MAS). We explored the variability within the LG3-PsMYB10 region using long-range sequences obtained in previous studies through CRISPR-Cas9 enrichment sequencing. We found that the PsMYB10. 2 gene was only expressed in red flesh fruits. Its role in promoting anthocyanin biosynthesis was validated by transient overexpression in Japanese plum fruits. The analysis of long-range sequences identified an LTR retrotransposon in the promoter of the expressed PsMYB10. 2 gene that explained the trait in 93. 1% of the 145 individuals analyzed. We hypothesize that the LTR retrotransposon may promote the PsMYB10. 2 expression and activate the anthocyanin biosynthesis pathway. We propose for the first time the PsMYB10. 2 gene as candidate for the flesh color natural variation in Japanese plum and provide a molecular marker for MAS.
Grants:	Ministerio de Economía, Industria y Competitividad BES-2016-079060 Agencia Estatal de Investigación RTI2018-100795-B-I00 Agencia Estatal de Investigación PID2021-128885OB-I00 Ministerio de Economía y Competitividad SEV-2015-0533 Agencia Estatal de Investigación CEX2019-000902-S
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Horticulture research, Vol. 9 (September 2022) , art. uhac206, ISSN 2052-7276

DOI: 10.1093/hr/uhac206
PMID: 36467274

12 p, 1.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
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