Use of a plasma focus device to study pulsed x-ray effects on peripheral blood lymphocytes : Analysis of chromosome aberrations
Verdejo, Valentina (Comisión Chilena de Energía Nuclear. Laboratorio Dosimetría Citogenética)
Radl, Analía (Comisión Chilena de Energía Nuclear. Laboratorio Dosimetría Citogenética)
Barquinero, Joan Francesc (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Jain, Jalaj (Comisión Chilena de Energía Nuclear. Centro de Investigación en la Intersección de Física de Plasmas, Materia y Complejidad)
Davis, Sergio (Comisión Chilena de Energía Nuclear. Centro de Investigación en la Intersección de Física de Plasmas, Materia y Complejidad)
Pavez, Cristian Arturo (Comisión Chilena de Energía Nuclear. Centro de Investigación en la Intersección de Física de Plasmas, Materia y Complejidad)
Soto, Leopoldo (Comisión Chilena de Energía Nuclear. Centro de Investigación en la Intersección de Física de Plasmas, Materia y Complejidad)
Moreno, José (Comisión Chilena de Energía Nuclear. Centro de Investigación en la Intersección de Física de Plasmas, Materia y Complejidad)

Data:	2023
Resum:	X-ray pulses (full width at half maximum ∼ 90 ns, dose rate ∼ 107 Gy s−1) were used to irradiate the monolayer of peripheral blood mononucleated cells using the PF-2kJ kilojoule plasma focus device. Four different exposure conditions were evaluated using 5, 10, 20, and 40 pulses, with the mean dose measured by TLD-100 being 0. 12 ± 0. 02 mGy, 0. 14 ± 0. 03 mGy, 0. 22 ± 0. 06 mGy, and 0. 47 ± 0. 09 mGy, respectively. Cytogenetic analysis showed an increase in all types of chromosomal aberrations following exposure to x-ray pulses. The distribution of dicentrics and centric rings was overdispersed after 5, 10, 20, and 40 pulses. Additionally, after 20 and 40 pulses, the presence of tricentric chromosomes is detected. Chromosome aberration frequencies found in this study were always higher than the estimated frequencies of chromosome aberrations using published dose-effect curves for conventional radiation sources. The overdispersion observed, the elevated maximum relative biological effectiveness (RBEM) and the presence of tricentric chromosomes at the relatively low doses of exposure (<0. 5 Gy) seem to indicate that low doses of pulsed x-rays of low energy show similar biological effects as those observed for high-LET radiation. X-ray pulses emitted by PF-2kJ were found to be more efficient in inducing chromosome aberrations, even more than α particles.
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Publicat a:	Journal of applied physics, Vol. 133 (April 2023) , art. 163302, ISSN 1089-7550

DOI: 10.1063/5.0141529

Postprint 34 p, 1.4 MB

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