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Pàgina inicial > Articles > Articles publicats > Effects of true-to-life PET nanoplastics using primary human nasal epithelial cells |
Data: | 2023 |
Resum: | Since inhalation is a relevant exposure route, studies using appropriate micro/nanoplastic (MNPLs) models, representative targeted cells, and relevant biomarkers of effect are required. We have used lab-made polyethylene terephthalate (PET)NPLs obtained from PET plastic water bottles. Human primary nasal epithelial cells (HNEpCs) were used as a model of the first barrier of the respiratory system. Cell internalization and intracellular reactive oxygen species (iROS) induction, as well as the effects on mitochondria functionality and in the modulation of the autophagy pathway, were evaluated. The data indicated significant cellular uptake and increased levels of iROS. Furthermore, a loss of mitochondrial membrane potential was observed in the exposed cells. Regarding the effects on the autophagy pathway, PETNPLs exposure significantly increases LC3-II protein expression levels. PETNPLs exposure also induced significant increases in the expression of p62. This is the first study showing that true-to-life PETNPLs can alter the autophagy pathway in HNEpCs. |
Ajuts: | Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731 Agencia Estatal de Investigación PID2020-116789-RB-C43 European Commission 965196 |
Nota: | Altres ajuts: acords transformatius de la UAB |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Nanoplastics ; PET ; HNEpCs ; Uptake ; Oxidative stress ; Mitochondrial membrane potential ; Autophagy |
Publicat a: | Environmental Toxicology and Pharmacology, Vol. 100 (June 2023) , art. 104140, ISSN 1872-7077 |
9 p, 4.6 MB |