Per citar aquest document: http://ddd.uab.cat/record/27763
Direct interaction between a human digestive protease and the mucoadhesive poly(acrylic acid)
Pallarès i Goitiz, Irantzu (Universitat Autponoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Fernández Fleischhauer, Daniel (Universitat Autponoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Comellas-Bigler, Mireia (Max-Planck Institute of Biochemistry. Proteinase Research Group)
Fernández-Recio, Juan (Barcelona Supercomputing Center. Life Sciences Department)
Ventura i Zamora, Salvador (Universitat Autponoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Avilés, Francesc X. (Francesc Xavier) (Universitat Autponoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Bode, Wolfram (Max-Planck Institute of Biochemistry. Proteinase Research Group)
Vendrell i Roca, Josep (Universitat Autponoma de Barcelona. Departament de Bioquímica i Biologia Molecular)

Data: 2008
Resum: Carboxypeptidase A1 has been the subject of extensive research in the last 30 y and is one of the most widely studied zinc metalloenzymes. However, the three-dimensional structure of the human form of the enzyme is not yet available. This report describes the three-dimensional structure of human carboxypeptidase A1 (hCPA1) derived from crystals that belong to the tetragonal space group P43212 and diffract to 1. 6 Å resolution. A description of the ternary complex hCPA1–Zn2+–poly(acrylic acid) is included as a model of the interaction of mucoadhesive polymers with proteases in the gastrointestinal tract. The direct mode of interaction between poly(acrylic acid) and the active site of the target protease was confirmed by in vitro inhibition assays. The structure was further analyzed in silico through the optimal docking-area method. The characterization of binding sites on the surface of hCPA1 and a comparison with other available carboxypeptidase structures provided further insights into the formation of multiprotein complexes and the activation mechanisms of carboxypeptidase zymogens. The high-resolution structure of hCPA1 provides an excellent template for the modelling of physiologically relevant carboxypeptidases and could also contribute to the design of specific agents for biomedical purposes.
Nota: Número d'acord de subvenció EC/FP6/018830
Nota: Número d'acord de subvenció MEC/BIO2004-05879
Nota: Número d'acord de subvenció MEC/BIO2005-06753
Nota: Número d'acord de subvenció MEC/BIO2007-68046
Nota: Número d'acord de subvenció AGAUR/2005/SGR-1037
Drets: Tots els drets reservats.
Llengua: Anglès.
Document: article ; publishedVersion
Matèria: Cristal·lografia ; Crystallography ; Carboxypeptidase A1 ; Mucoadhesive polymers ; Zinc metalloenzymes ; Digestive proteases
Publicat a: Acta crystallographica. Section D, Biological crystallography, Vol. 64, Issue 7 (July 2008) , p. 784-791, ISSN 1399-0047

DOI: 10.1107/S0907444908013474


8 p, 1.0 MB

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