Guillain-Barré Syndrome Following Zika Virus Infection Is Associated With a Diverse Spectrum of Peripheral Nerve Reactive Antibodies
Davies, Alexander J.
Lleixà, Cinta (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Siles, Ana Maria (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Gourlay, Dawn S.
Berridge, Georgina
Dejnirattisai, Wanwisa
Ramírez-Santana, Carolina
Anaya, Juan-Manuel
Falconar, Andrew K.
Romero-Vivas, Claudia M.
Osorio, Lyda
Parra, Beatriz
Screaton, Gavin R.
Mongkolsapaya, Juthathip
Fischer, Roman
Pardo, Carlos A.
Halstead, Susan K.
Willison, Hugh J.
Querol, Luis (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Rinaldi, Simon
Universitat Autònoma de Barcelona

Data:	2022
Resum:	Recent outbreaks of Zika virus (ZIKV) in South and Central America have highlighted significant neurologic side effects. Concurrence with the inflammatory neuropathy Guillain-Barré syndrome (GBS) is observed in 1:4,000 ZIKV cases. Whether the neurologic symptoms of ZIKV infection are immune mediated is unclear. We used rodent and human live cellular models to screen for anti-peripheral nerve reactive IgG and IgM autoantibodies in the sera of patients with ZIKV with and without GBS. In this study, 52 patients with ZIKV-GBS were compared with 134 ZIKV-infected patients without GBS and 91 non-ZIKV controls. Positive sera were taken forward for target identification by immunoprecipitation and mass spectrometry, and candidate antigens were validated by ELISA and cell-based assays. Autoantibody reactions against glycolipid antigens were also screened on an array. Overall, IgG antibody reactivities to rat Schwann cells (SCs) (6. 5%) and myelinated cocultures (9. 6%) were significantly higher, albeit infrequent, in the ZIKV-GBS group compared with all controls. IgM antibody immunoreactivity to dorsal root ganglia neurones (32. 3%) and SCs (19. 4%) was more frequently observed in the ZIKV-GBS group compared with other controls, whereas IgM reactivity to cocultures was as common in ZIKV and non-ZIKV sera. Strong axonal-binding ZIKV-GBS serum IgG antibodies from 1 patient were confirmed to react with neurofascin 155 and 186. Serum from a ZIKV-infected patient without GBS displayed strong myelin-binding and putative antilipid antigen reaction characteristics. There was, however, no significant association of ZIKV-GBS with any known antiglycolipid antibodies. Autoantibody responses in ZIKV-GBS target heterogeneous peripheral nerve antigens suggesting heterogeneity of the humoral immune response despite a common prodromal infection.
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Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Neurology® neuroimmunology & neuroinflammation, Vol. 10 (november 2022) , ISSN 2332-7812

DOI: 10.1212/NXI.0000000000200047
PMID: 36411078

14 p, 917.7 KB

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