Graphene Oxide Nanosheets Reduce Astrocyte Reactivity to Inflammation and Ameliorate Experimental Autoimmune Encephalomyelitis
Di Mauro, Giuseppe (Scuola Internazionale Superiore di Studi Avanzati)
Amoriello, Roberta (University of Florence)
Lozano, Neus (Institut Català de Nanociència i Nanotecnologia)
Carnasciali, Alberto (University of Florence)
Guasti, Daniele (University of Florence)
Becucci, Maurizio (University of Florence)
Cellot, Giada (Scuola Internazionale Superiore di Studi Avanzati)
Kostarelos, Kostas (Institut Català de Nanociència i Nanotecnologia)
Ballerini, Clara (University of Florence)
Ballerini, Laura (Scuola Internazionale Superiore di Studi Avanzati)

Data:	2023
Resum:	In neuroinflammation, astrocytes play multifaceted roles that regulate the neuronal environment. Astrocytes sense and respond to pro-inflammatory cytokines (CKs) and, by a repertoire of intracellular Ca signaling, contribute to disease progression. Therapeutic approaches wish to reduce the overactivation in Ca signaling in inflammatory-reactive astrocytes to restore dysregulated cellular changes. Cell-targeting therapeutics might take advantage by the use of nanomaterial-multifunctional platforms such as graphene oxide (GO). GO biomedical applications in the nervous system involve therapeutic delivery and sensing, and GO flakes were shown to enable interfacing of neuronal and glial membrane dynamics. We exploit organotypic spinal cord cultures and optical imaging to explore Ca changes in astrocytes, and we report, when spinal tissue is exposed to CKs, neuroinflammatory-associated modulation of resident glia. We show the efficacy of GO to revert these dynamic changes in astrocytic reactivity to CKs, and we translate this potential in an animal model of immune-mediated neuroinflammatory disease.
Ajuts:	European Commission 881603 Agencia Estatal de Investigación SEV-2017-0706
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	ACS nano, Vol. 17, Issue 3 (February 2023) , p. 1965-1978, ISSN 1936-086X

DOI: 10.1021/acsnano.2c06609
PMID: 36692902

14 p, 2.2 MB

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