Lung Persistence, Biodegradation, and Elimination of Graphene-Based Materials are Predominantly Size-Dependent and Mediated by Alveolar Phagocytes
Loret, Thomas (University of Manchester. Nanomedicine Lab)
De Luna, Luis Augusto Visani (Manchester Academic Health Science Centre)
Lucherelli, Matteo Andrea (University of Strasbourg)
Fordham, Alexander (University of Manchester. Nanomedicine Lab)
Lozano, Neus (Institut Català de Nanociència i Nanotecnologia)
Bianco, Alberto (University of Strasbourg)
Kostarelos, Kostas (Institut Català de Nanociència i Nanotecnologia)
Bussy, Cyrill (University of Manchester. Nanomedicine Lab)

Data:	2023
Resum:	Graphene-based materials (GBMs) have promising applications in various sectors, including pulmonary nanomedicine. Nevertheless, the influence of GBM physicochemical characteristics on their fate and impact in lung has not been thoroughly addressed. To fill this gap, the biological response, distribution, and bio-persistence of four different GBMs in mouse lungs up to 28 days after single oropharyngeal aspiration are investigated. None of the GBMs, varying in size (large versus small) and carbon to oxygen ratio as well as thickness (few-layers graphene (FLG) versus thin graphene oxide (GO)), induce a strong pulmonary immune response. However, recruited neutrophils internalize nanosheets better and degrade GBMs faster than macrophages, revealing their crucial role in the elimination of small GBMs. In contrast, large GO sheets induce more damages due to a hindered degradation and long-term persistence in macrophages. Overall, small dimensions appear to be a leading feature in the design of safe GBM pulmonary nanovectors due to an enhanced degradation in phagocytes and a faster clearance from the lungs for small GBMs. Thickness also plays an important role, since decreased material loading in alveolar phagocytes and faster elimination are found for FLGs compared to thinner GOs. These results are important for designing safer-by-design GBMs for biomedical application.
Ajuts:	Ministerio de Ciencia e Innovación CEX2021-001214-S Agencia Estatal de Investigación SEV-2017-0706 European Commission 785219 European Commission 881603
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Small, Vol. 19, Issue 39 (September 2023) , art. 2301201, ISSN 1613-6829

DOI: 10.1002/smll.202301201

17 p, 11.3 MB

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