Glycoprotein profile assessed by1h-nmr as a global inflammation marker in patients with HIV infection. A prospective study
Malo, Ana-Irene (Universitat Rovira i Virgili)
Rull, Anna (Universitat Rovira i Virgili)
Girona, Josefa (Universitat Rovira i Virgili)
Domingo, Pere (Institut d'Investigació Biomèdica Sant Pau)
Fuertes-Martín, Rocío (Universitat Rovira i Virgili)
Amigó, Núria (Universitat Rovira i Virgili)
Rodríguez-Borjabad, Cèlia (Universitat Rovira i Virgili)
Martínez-Micaelo, Neus (Universitat Rovira i Virgili)
Leal, Manuel (Viamed-Santa Ángela de la Cruz Hospital)
Peraire, Joaquim (Universitat Rovira i Virgili)
Correig, Xavier (Universitat Rovira i Virgili)
Vidal, Francesc (Universitat Rovira i Virgili)
Masana, Luis (Universitat Rovira i Virgili)
Universitat Autònoma de Barcelona

Fecha:	2020
Resumen:	Plasma glycoproteins are a composite biomarker of inflammation and can be detected byH-NMR. The aim of this study was to prospectively appraise the clinical value of plasma glycopro-teins assessed byH-NMR in people living with HIV (PLWH). A total of 221 patients with HIV infection were recruited and studied at baseline and at 48 and 144 weeks. Patients were distributed into two groups according to baseline CD4 T-cell number below or above 200 cells/µL. Patients with fewer than 200 cells/µL were distributed into the responders and nonresponders according to antiretroviral therapy (ART) response at 144 weeks. Glycoprotein concentrations were determined byH-NMR arising from the protein bond N-acetylglucosamine and N-acetylgalactosamine signals (GlycA); and N-acetylneuraminic acid signal (GlycB) associated with the sugar-protein bond con-centration and aggregation state (shapes (height/width)). Basal glycoprotein concentrations were higher in patients with < 200 CD4 T-cell/µL (Glyc A: 1040(917. 9-1199. 1) vs. 950. 4(845. 5-1050. 9), p < 0. 001, and Glyc B: 521(440. 3-610. 3) vs. 468. 6(417. 9-507. 0) µ mol/L, p < 0. 001) being reduced by ART. The height/width (H/W) ratio was the parameter showing a better association with this clinical sta-tus. Baseline glycoproteins predict the condition of responder/nonresponder. In this study,H-NMR glycoproteins provide novel insights to assess inflammation status and have prognostic value in PLWH.
Ayudas:	Ministerio de Economía y Competitividad PI13/00796 Ministerio de Economía y Competitividad PI16/00503 Instituto de Salud Carlos III PI19/01337 Ministerio de Economía y Competitividad RD16/0025/0006
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Glycoprotein ; HIV-1 ; Immunological responders ; Inflammation ; Proton nuclear magnetic resonance (1H-NMR)
Publicado en:	Journal of clinical medicine, Vol. 9 Núm. 5 (may 2020) , p. 1344, ISSN 2077-0383

DOI: 10.3390/jcm9051344
PMID: 32375373

12 p, 2.1 MB

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