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Página principal > Artículos > Artículos publicados > Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
Fecha: | 2022 |
Resumen: | The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1. 6 was obtained for 100% of isolates in 5 laboratories (68. 2-98. 6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification. |
Ayudas: | Ministerio de Economía y Competitividad PI15/01073 Instituto de Salud Carlos III PI18/00997 Instituto de Salud Carlos III PI18/01068 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió publicada |
Materia: | Clinical microbiology ; Infectious-disease diagnostics |
Publicado en: | Scientific reports, Vol. 12 Núm. 1 (december 2022) , p. 1237, ISSN 2045-2322 |
7 p, 1.0 MB |