Published articles

Search collections:

Sort by:	Display results:	Output format:

Published articles	1 records found	Search took 0.00 seconds.

15 p, 1.7 MB

Selecting subpopulations of high-quality protein conformers among conformational mixtures of recombinant bovine MMP-9 solubilized from inclusion bodies / Carratalá, José Vicente (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Gifre-Renom, Laia (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Producció de Remugants) ; Roca-Pinilla, Ramon (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Producció de Remugants) ; Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") ; Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Producció de Remugants) ; Garcia-Fruitos, Elena (Institut de Recerca i Tecnologia Agroalimentàries. Departament de Producció de Remugants) ; Sanchez, Julieta M. (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) ; Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies-IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories Clearcoli (an engineered form of Escherichia coli) and Lactococcus lactis, mainly forming part of IBs and partially recovered under non-denaturing conditions. [...]
2021 - DOI (Digital Object Identifier)

10.3390/ijms22063020
International journal of molecular sciences, Vol. 22, Issue 6 (March 2021) , art. 3020

Detailed record -

Interested in being notified about new results for this query?
Set up a personal email alert or subscribe to the RSS feed.