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PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping / Molina-Moya, Barbara (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Lacoma, Alicia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; García Sierra, N. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Blanco, S. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Haba, L. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Samper, S. (Centro de Investigación Biomédica en Red de Enfermedades Respiratorias) ; Ruiz Manzano, J.. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Prat, C. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol) ; Arnold, C. (Genomic Services and Development Unit, Public Health England) ; Domínguez, J.. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units - variable number tandem repeats (MIRU-VNTR), and PyroTyping. [...]
2017 - DOI (Digital Object Identifier)

10.1038/s41598-017-06760-5
Scientific reports, Vol. 7 Núm. 6777 (july 2017) , p. 1-7

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