Web of Science: 19 citas, Scopus: 22 citas, Google Scholar: citas
A Novel Liposome-Based Nanocarrier Loaded with an LPS-dsRNA Cocktail for Fish Innate Immune System Stimulation
Ruyra Ripoll, Àngels (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Cano Sarabia, Antonia María (Institut Català de Nanociència i Nanotecnologia)
Mackenzie, Simon A. (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Maspoch Comamala, Daniel (Institut Català de Nanociència i Nanotecnologia)
Roher Armentia, Nerea (Universitat Autònoma de Barcelona. Departament de Biologia, de Fisiologia i d'Immunologia)

Fecha: 2013
Resumen: Development of novel systems of vaccine delivery is a growing demand of the aquaculture industry. Nano- and micro- encapsulation systems are promising tools to achieve efficient vaccines against orphan vaccine fish diseases. In this context, the use of liposomal based-nanocarriers has been poorly explored in fish; although liposomal nanocarriers have successfully been used in other species. Here, we report a new ~125 nm-in-diameter unilamellar liposome-encapsulated immunostimulant cocktail containing crude lipopolysaccharide (LPS) from E. coli and polyinosinic:polycytidylic acid [poly (I:C)], a synthetic analog of dsRNA virus, aiming to be used as a non-specific vaccine nanocarrier in different fish species. This liposomal carrier showed high encapsulation efficiencies and low toxicity not only in vitro using three different cellular models but also in vivo using zebrafish embryos and larvae. We showed that such liposomal LPS-dsRNA cocktail is able to enter into contact with zebrafish hepatocytes (ZFL cell line) and trout macrophage plasma membranes, being preferentially internalized through caveolae-dependent endocytosis, although clathrin-mediated endocytosis in ZFL cells and macropinocytocis in macrophages also contribute to liposome uptake. Importantly, we also demonstrated that this liposomal LPS-dsRNA cocktail elicits a specific pro-inflammatory and anti-viral response in both zebrafish hepatocytes and trout macrophages. The design of a unique delivery system with the ability to stimulate two potent innate immunity pathways virtually present in all fish species represents a completely new approach in fish health.
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès.
Documento: article ; recerca ; publishedVersion
Materia: Liposomes ; Macrophages ; Trout ; Endocytosis ; Toxicity ; Zebrafish ; Fluorescence imaging ; Larvae
Publicado en: PLoS one, Vol. 8 Issue 10 (October 2013) , p. e76338, ISSN 1932-6203

DOI: 10.1371/journal.pone.0076338
PMID: 24204616


13 p, 3.9 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
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 Registro creado el 2015-10-20, última modificación el 2019-05-10



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