DNA polymorphism sensitive impedimetric detection on gold-nanoislands modified electrodes
Bonanni, Alessandra (Nanyang Technological University. School of Physical and Mathematical Sciences)
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química)
Valle Zafra, Manuel del (Universitat Autònoma de Barcelona. Departament de Química)

Fecha:	2015
Resumen:	Nanocomposite materials are being increasingly used in biosensing applications as they can significantly improve biosensor performance. Here we report the use of a novel impedimetric genosensor based on gold nanoparticles graphite-epoxy nanocomposite (nanoAu-GEC) for the detection of triple base mutation deletion in a cystic-fibrosis (CF) related human DNA sequence. The developed platform consists of chemisorbing gold nano-islands surrounded by rigid, non-chemisorbing, and conducting graphite-epoxy composite. The ratio of the gold nanoparticles in the composite was carefully optimized by electrochemical and microscopy studies. Such platform allows the very fast and stable thiol immobilization of DNA probes on the gold islands, thus minimizing the steric and electrostatic repulsion among the DNA probes and improving the detection of DNA polymorphism down to 2. 25 fmol by using electrochemical impedance spectroscopy. These findings are very important in order to develop new and renewable platforms to be used in point-of-care devices for the detection of biomolecules.
Ayudas:	Ministerio de Economía y Competitividad CTQ2013-41577-P Ministerio de Economía y Competitividad BIO2010-17566
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Gold nanocomposite ; DNA polymorphism ; Electrochemical impedance spectroscopy ; Cyctic fibrosis ; Gold nanoparticles amplification
Publicado en:	Talanta, Vol. 136 (May 2015) , p. 95-101, ISSN 1873-3573

DOI: 10.1016/j.talanta.2015.01.004

Post-print 23 p, 6.5 MB

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